1ttu
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1ttu.gif|left|200px]] | + | [[Image:1ttu.gif|left|200px]] |
| - | + | ||
| - | '''Crystal Structure of CSL bound to DNA''' | + | {{Structure |
| + | |PDB= 1ttu |SIZE=350|CAPTION= <scene name='initialview01'>1ttu</scene>, resolution 2.85Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= lag-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of CSL bound to DNA''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1TTU is a [ | + | 1TTU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TTU OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the nuclear effector of Notch signaling, CSL, bound to DNA., Kovall RA, Hendrickson WA, EMBO J. 2004 Sep 1;23(17):3441-51. Epub 2004 Aug 5. PMID:[http:// | + | Crystal structure of the nuclear effector of Notch signaling, CSL, bound to DNA., Kovall RA, Hendrickson WA, EMBO J. 2004 Sep 1;23(17):3441-51. Epub 2004 Aug 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15297877 15297877] |
[[Category: Caenorhabditis elegans]] | [[Category: Caenorhabditis elegans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 23: | Line 32: | ||
[[Category: transcription factor]] | [[Category: transcription factor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:23:04 2008'' |
Revision as of 12:23, 20 March 2008
| |||||||
| , resolution 2.85Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | lag-1 (Caenorhabditis elegans) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of CSL bound to DNA
Overview
Notch signaling is a conserved pathway of communication between neighboring cells that results in cell fate specification, and CSL is the universal transcriptional effector of Notch signaling. The Notch intracellular domain translocates to the nucleus after proteolytic release upon Notch extracellular engagement, and there it displaces corepressors from DNA-bound CSL and recruits activators of Notch target genes. Here we report the 2.85 A crystal structure of CSL with a target DNA. CSL comprises three structurally integrated domains: its amino (NTD)- and carboxy (CTD)-terminal domains are strikingly similar to those of Rel transcription factors, but a surprising beta-trefoil domain (BTD) is inserted between them. CSL-bound DNA is recognized specifically by conserved residues from NTD and BTD. A hydrophobic pocket on BTD is identified as the likely site of Notch interaction with CSL, which has functional implications for the mechanism of Notch signaling.
About this Structure
1TTU is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.
Reference
Crystal structure of the nuclear effector of Notch signaling, CSL, bound to DNA., Kovall RA, Hendrickson WA, EMBO J. 2004 Sep 1;23(17):3441-51. Epub 2004 Aug 5. PMID:15297877
Page seeded by OCA on Thu Mar 20 14:23:04 2008
