1v0d

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[[Image:1v0d.gif|left|200px]]<br /><applet load="1v0d" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1v0d.gif|left|200px]]
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caption="1v0d, resolution 2.6&Aring;" />
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'''CRYSTAL STRUCTURE OF CASPASE-ACTIVATED DNASE (CAD)'''<br />
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{{Structure
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|PDB= 1v0d |SIZE=350|CAPTION= <scene name='initialview01'>1v0d</scene>, resolution 2.6&Aring;
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|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+A'>AC1</scene>
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=PB:LEAD (II) ION'>PB</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''CRYSTAL STRUCTURE OF CASPASE-ACTIVATED DNASE (CAD)'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1V0D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=PB:'>PB</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1V0D with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb56_1.html Caspases]]. Known structural/functional Site: <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V0D OCA].
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1V0D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. The following page contains interesting information on the relation of 1V0D with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb56_1.html Caspases]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V0D OCA].
==Reference==
==Reference==
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Structural mechanism for inactivation and activation of CAD/DFF40 in the apoptotic pathway., Woo EJ, Kim YG, Kim MS, Han WD, Shin S, Robinson H, Park SY, Oh BH, Mol Cell. 2004 May 21;14(4):531-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15149602 15149602]
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Structural mechanism for inactivation and activation of CAD/DFF40 in the apoptotic pathway., Woo EJ, Kim YG, Kim MS, Han WD, Shin S, Robinson H, Park SY, Oh BH, Mol Cell. 2004 May 21;14(4):531-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15149602 15149602]
[[Category: Caspases]]
[[Category: Caspases]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: nuclease]]
[[Category: nuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:30:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:39:17 2008''

Revision as of 12:39, 20 March 2008

Image:1v0d.gif


PDB ID 1v0d

Drag the structure with the mouse to rotate
, resolution 2.6Å
Sites:
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF CASPASE-ACTIVATED DNASE (CAD)


Overview

CAD/DFF40 is responsible for the degradation of chromosomal DNA into nucleosomal fragments and subsequent chromatin condensation during apoptosis. It exists as an inactive complex with its inhibitor ICAD/DFF45 in proliferating cells but becomes activated upon cleavage of ICAD/DFF45 into three domains by caspases in dying cells. The molecular mechanism underlying the control and activation of CAD/DFF40 was unknown. Here, the crystal structure of activated CAD/DFF40 reveals that it is a pair of molecular scissors with a deep active-site crevice that appears ideal for distinguishing internucleosomal DNA from nucleosomal DNA. Ensuing studies show that ICAD/DFF45 sequesters the nonfunctional CAD/DFF40 monomer and is also able to disassemble the functional CAD/DFF40 dimer. This capacity requires the involvement of the middle domain of ICAD/DFF45, which by itself cannot remain bound to CAD/DFF40 due to low binding affinity for the enzyme. Thus, the consequence of the caspase-cleavage of ICAD/DFF45 is a self-assembly of CAD/DFF40 into the active dimer.

About this Structure

1V0D is a Single protein structure of sequence from Mus musculus. The following page contains interesting information on the relation of 1V0D with [Caspases]. Full crystallographic information is available from OCA.

Reference

Structural mechanism for inactivation and activation of CAD/DFF40 in the apoptotic pathway., Woo EJ, Kim YG, Kim MS, Han WD, Shin S, Robinson H, Park SY, Oh BH, Mol Cell. 2004 May 21;14(4):531-9. PMID:15149602

Page seeded by OCA on Thu Mar 20 14:39:17 2008

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