1v1h
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1v1h.gif|left|200px]] | + | [[Image:1v1h.gif|left|200px]] |
| - | + | ||
| - | '''ADENOVIRUS FIBRE SHAFT SEQUENCE N-TERMINALLY FUSED TO THE BACTERIOPHAGE T4 FIBRITIN FOLDON TRIMERISATION MOTIF WITH A SHORT LINKER''' | + | {{Structure |
| + | |PDB= 1v1h |SIZE=350|CAPTION= <scene name='initialview01'>1v1h</scene>, resolution 1.9Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''ADENOVIRUS FIBRE SHAFT SEQUENCE N-TERMINALLY FUSED TO THE BACTERIOPHAGE T4 FIBRITIN FOLDON TRIMERISATION MOTIF WITH A SHORT LINKER''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1V1H is a [ | + | 1V1H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_adenovirus_type_2,_bacteriophage_t4 Human adenovirus type 2, bacteriophage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V1H OCA]. |
==Reference== | ==Reference== | ||
| - | Adenovirus fibre shaft sequences fold into the native triple beta-spiral fold when N-terminally fused to the bacteriophage T4 fibritin foldon trimerisation motif., Papanikolopoulou K, Teixeira S, Belrhali H, Forsyth VT, Mitraki A, van Raaij MJ, J Mol Biol. 2004 Sep 3;342(1):219-27. PMID:[http:// | + | Adenovirus fibre shaft sequences fold into the native triple beta-spiral fold when N-terminally fused to the bacteriophage T4 fibritin foldon trimerisation motif., Papanikolopoulou K, Teixeira S, Belrhali H, Forsyth VT, Mitraki A, van Raaij MJ, J Mol Biol. 2004 Sep 3;342(1):219-27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15313619 15313619] |
[[Category: Human adenovirus type 2, bacteriophage t4]] | [[Category: Human adenovirus type 2, bacteriophage t4]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 23: | Line 32: | ||
[[Category: fiber protein]] | [[Category: fiber protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:39:38 2008'' |
Revision as of 12:39, 20 March 2008
| |||||||
| , resolution 1.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ADENOVIRUS FIBRE SHAFT SEQUENCE N-TERMINALLY FUSED TO THE BACTERIOPHAGE T4 FIBRITIN FOLDON TRIMERISATION MOTIF WITH A SHORT LINKER
Overview
Adenovirus fibres are trimeric proteins that consist of a globular C-terminal domain, a central fibrous shaft and an N-terminal part that attaches to the viral capsid. In the presence of the globular C-terminal domain, which is necessary for correct trimerisation, the shaft segment adopts a triple beta-spiral conformation. We have replaced the head of the fibre by the trimerisation domain of the bacteriophage T4 fibritin, the foldon. Two different fusion constructs were made and crystallised, one with an eight amino acid residue linker and one with a linker of only two residues. X-ray crystallographic studies of both fusion proteins shows that residues 319-391 of the adenovirus type 2 fibre shaft fold into a triple beta-spiral fold indistinguishable from the native structure, although this is now resolved at a higher resolution of 1.9 A. The foldon residues 458-483 also adopt their natural structure. The intervening linkers are not well ordered in the crystal structures. This work shows that the shaft sequences retain their capacity to fold into their native beta-spiral fibrous fold when fused to a foreign C-terminal trimerisation motif. It provides a structural basis to artificially trimerise longer adenovirus shaft segments and segments from other trimeric beta-structured fibre proteins. Such artificial fibrous constructs, amenable to crystallisation and solution studies, can offer tractable model systems for the study of beta-fibrous structure. They can also prove useful for gene therapy and fibre engineering applications.
About this Structure
1V1H is a Single protein structure of sequence from Human adenovirus type 2, bacteriophage t4. Full crystallographic information is available from OCA.
Reference
Adenovirus fibre shaft sequences fold into the native triple beta-spiral fold when N-terminally fused to the bacteriophage T4 fibritin foldon trimerisation motif., Papanikolopoulou K, Teixeira S, Belrhali H, Forsyth VT, Mitraki A, van Raaij MJ, J Mol Biol. 2004 Sep 3;342(1):219-27. PMID:15313619
Page seeded by OCA on Thu Mar 20 14:39:38 2008
