1vdd
From Proteopedia
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| - | [[Image:1vdd.gif|left|200px]] | + | [[Image:1vdd.gif|left|200px]] |
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| - | '''Crystal structure of recombinational repair protein RecR''' | + | {{Structure |
| + | |PDB= 1vdd |SIZE=350|CAPTION= <scene name='initialview01'>1vdd</scene>, resolution 2.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= recR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 Deinococcus radiodurans]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of recombinational repair protein RecR''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1VDD is a [ | + | 1VDD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VDD OCA]. |
==Reference== | ==Reference== | ||
| - | Ring-shaped architecture of RecR: implications for its role in homologous recombinational DNA repair., Lee BI, Kim KH, Park SJ, Eom SH, Song HK, Suh SW, EMBO J. 2004 May 19;23(10):2029-38. Epub 2004 Apr 29. PMID:[http:// | + | Ring-shaped architecture of RecR: implications for its role in homologous recombinational DNA repair., Lee BI, Kim KH, Park SJ, Eom SH, Song HK, Suh SW, EMBO J. 2004 May 19;23(10):2029-38. Epub 2004 Apr 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15116069 15116069] |
[[Category: Deinococcus radiodurans]] | [[Category: Deinococcus radiodurans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: zinc finger]] | [[Category: zinc finger]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:44:05 2008'' |
Revision as of 12:44, 20 March 2008
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| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | recR (Deinococcus radiodurans) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of recombinational repair protein RecR
Overview
RecR, together with RecF and RecO, facilitates RecA loading in the RecF pathway of homologous recombinational DNA repair in procaryotes. The human Rad52 protein is a functional counterpart of RecFOR. We present here the crystal structure of RecR from Deinococcus radiodurans (DR RecR). A monomer of DR RecR has a two-domain structure: the N-terminal domain with a helix-hairpin-helix (HhH) motif and the C-terminal domain with a Cys4 zinc-finger motif, a Toprim domain and a Walker B motif. Four such monomers form a ring-shaped tetramer of 222 symmetry with a central hole of 30-35 angstroms diameter. In the crystal, two tetramers are concatenated, implying that the RecR tetramer is capable of opening and closing. We also show that DR RecR binds to both dsDNA and ssDNA, and that its HhH motif is essential for DNA binding.
About this Structure
1VDD is a Single protein structure of sequence from Deinococcus radiodurans. Full crystallographic information is available from OCA.
Reference
Ring-shaped architecture of RecR: implications for its role in homologous recombinational DNA repair., Lee BI, Kim KH, Park SJ, Eom SH, Song HK, Suh SW, EMBO J. 2004 May 19;23(10):2029-38. Epub 2004 Apr 29. PMID:15116069
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