1w0n

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1w0n.jpg|left|200px]]<br /><applet load="1w0n" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1w0n.jpg|left|200px]]
-
caption="1w0n, resolution 0.80&Aring;" />
+
 
-
'''STRUCTURE OF UNCOMPLEXED CARBOHYDRATE BINDING DOMAIN CBM36'''<br />
+
{{Structure
 +
|PDB= 1w0n |SIZE=350|CAPTION= <scene name='initialview01'>1w0n</scene>, resolution 0.80&Aring;
 +
|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+A'>AC1</scene>
 +
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]
 +
|GENE=
 +
}}
 +
 
 +
'''STRUCTURE OF UNCOMPLEXED CARBOHYDRATE BINDING DOMAIN CBM36'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1W0N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paenibacillus_polymyxa Paenibacillus polymyxa] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] Known structural/functional Site: <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W0N OCA].
+
1W0N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Paenibacillus_polymyxa Paenibacillus polymyxa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W0N OCA].
==Reference==
==Reference==
-
Ab initio structure determination and functional characterization of CBM36; a new family of calcium-dependent carbohydrate binding modules., Jamal-Talabani S, Boraston AB, Turkenburg JP, Tarbouriech N, Ducros VM, Davies GJ, Structure. 2004 Jul;12(7):1177-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15242594 15242594]
+
Ab initio structure determination and functional characterization of CBM36; a new family of calcium-dependent carbohydrate binding modules., Jamal-Talabani S, Boraston AB, Turkenburg JP, Tarbouriech N, Ducros VM, Davies GJ, Structure. 2004 Jul;12(7):1177-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15242594 15242594]
[[Category: Endo-1,4-beta-xylanase]]
[[Category: Endo-1,4-beta-xylanase]]
[[Category: Paenibacillus polymyxa]]
[[Category: Paenibacillus polymyxa]]
Line 25: Line 34:
[[Category: xylanase]]
[[Category: xylanase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:39:14 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:50:32 2008''

Revision as of 12:50, 20 March 2008

Image:1w0n.jpg


PDB ID 1w0n

Drag the structure with the mouse to rotate
, resolution 0.80Å
Sites:
Ligands: , and
Activity: Endo-1,4-beta-xylanase, with EC number 3.2.1.8
Coordinates: save as pdb, mmCIF, xml



STRUCTURE OF UNCOMPLEXED CARBOHYDRATE BINDING DOMAIN CBM36


Overview

The enzymatic degradation of polysaccharides harnesses multimodular enzymes whose carbohydrate binding modules (CBM) target the catalytic domain onto the recalcitrant substrate. Here we report the ab initio structure determination and subsequent refinement, at 0.8 A resolution, of the CBM36 domain of the Paenibacillus polymyxa xylanase 43A. Affinity electrophoresis, isothermal titration calorimetry, and UV difference spectroscopy demonstrate that CBM36 is a novel Ca(2+)-dependent xylan binding domain. The 3D structure of CBM36 in complex with xylotriose and Ca(2+), at 1.5 A resolution, displays significant conformational changes compared to the native structure and reveals the molecular basis for its unique Ca(2+)-dependent binding of xylooligosaccharides through coordination of the O2 and O3 hydroxyls. CBM36 is one of an emerging spectrum of carbohydrate binding modules that increasingly find applications in industry and display great potential for mapping the "glyco-architecture" of plant cells.

About this Structure

1W0N is a Single protein structure of sequence from Paenibacillus polymyxa. Full crystallographic information is available from OCA.

Reference

Ab initio structure determination and functional characterization of CBM36; a new family of calcium-dependent carbohydrate binding modules., Jamal-Talabani S, Boraston AB, Turkenburg JP, Tarbouriech N, Ducros VM, Davies GJ, Structure. 2004 Jul;12(7):1177-87. PMID:15242594

Page seeded by OCA on Thu Mar 20 14:50:32 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1w0n"
Personal tools