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3puk
From Proteopedia
(Difference between revisions)
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| - | + | ==Re-refinement of the crystal structure of Munc18-3 and Syntaxin4 N-peptide complex== | |
| - | + | <StructureSection load='3puk' size='340' side='right' caption='[[3puk]], [[Resolution|resolution]] 3.05Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[3puk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2pjx 2pjx]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PUK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PUK FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2pjx|2pjx]], [[3puj|3puj]]</td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Stxbp3, Stxbp3a, Unc18c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3puk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3puk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3puk RCSB], [http://www.ebi.ac.uk/pdbsum/3puk PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/STXB3_MOUSE STXB3_MOUSE]] Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes.<ref>PMID:9045631</ref> [[http://www.uniprot.org/uniprot/STX4_MOUSE STX4_MOUSE]] Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes and in docking of synaptic vesicles at presynaptic active zones.<ref>PMID:9045631</ref> <ref>PMID:10394363</ref> <ref>PMID:18827011</ref> | [[http://www.uniprot.org/uniprot/STXB3_MOUSE STXB3_MOUSE]] Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes.<ref>PMID:9045631</ref> [[http://www.uniprot.org/uniprot/STX4_MOUSE STX4_MOUSE]] Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes and in docking of synaptic vesicles at presynaptic active zones.<ref>PMID:9045631</ref> <ref>PMID:10394363</ref> <ref>PMID:18827011</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Munc18-1 and Syntaxin1 are essential proteins for SNARE-mediated neurotransmission. Munc18-1 participates in synaptic vesicle fusion via dual roles: as a docking/chaperone protein by binding closed Syntaxin1, and as a fusion protein that binds SNARE complexes in a Syntaxin1 N-peptide dependent manner. The two roles are associated with a closed-open Syntaxin1 conformational transition. Here, we show that Syntaxin N-peptide binding to Munc18-1 is not highly selective, suggesting that other parts of the SNARE complex are involved in binding to Munc18-1. We also find that Syntaxin1, with an N peptide and a physically anchored C terminus, binds to Munc18-1 and that this complex can participate in SNARE complex formation. We report a Munc18-1-N-peptide crystal structure that, together with other data, reveals how Munc18-1 might transit from a conformation that binds closed Syntaxin1 to one that may be compatible with binding open Syntaxin1 and SNARE complexes. Our results suggest the possibility that structural transitions occur in both Munc18-1 and Syntaxin1 during their binary interaction. We hypothesize that Munc18-1 domain 3a undergoes a conformational change that may allow coiled-coil interactions with SNARE complexes. | ||
| - | + | Possible roles for Munc18-1 domain 3a and Syntaxin1 N-peptide and C-terminal anchor in SNARE complex formation.,Hu SH, Christie MP, Saez NJ, Latham CF, Jarrott R, Lua LH, Collins BM, Martin JL Proc Natl Acad Sci U S A. 2010 Dec 30. PMID:21193638<ref>PMID:21193638</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Lk3 transgenic mice]] | [[Category: Lk3 transgenic mice]] | ||
| - | [[Category: Christie, M P | + | [[Category: Christie, M P]] |
| - | [[Category: Collins, B M | + | [[Category: Collins, B M]] |
| - | [[Category: Hu, S H | + | [[Category: Hu, S H]] |
| - | [[Category: Jarrott, R | + | [[Category: Jarrott, R]] |
| - | [[Category: Latham, C F | + | [[Category: Latham, C F]] |
| - | [[Category: Lua, L H.L | + | [[Category: Lua, L H.L]] |
| - | [[Category: Martin, J L | + | [[Category: Martin, J L]] |
| - | [[Category: Saez, N J | + | [[Category: Saez, N J]] |
[[Category: Endocytosis-exocytosis complex]] | [[Category: Endocytosis-exocytosis complex]] | ||
[[Category: Membrane trafficking]] | [[Category: Membrane trafficking]] | ||
Revision as of 08:52, 24 December 2014
Re-refinement of the crystal structure of Munc18-3 and Syntaxin4 N-peptide complex
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