This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1wrm

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1wrm.gif|left|200px]]<br /><applet load="1wrm" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1wrm.gif|left|200px]]
-
caption="1wrm, resolution 1.50&Aring;" />
+
 
-
'''Crystal structure of JSP-1'''<br />
+
{{Structure
 +
|PDB= 1wrm |SIZE=350|CAPTION= <scene name='initialview01'>1wrm</scene>, resolution 1.50&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC ACID'>MES</scene>
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48]
 +
|GENE=
 +
}}
 +
 
 +
'''Crystal structure of JSP-1'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1WRM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MES:'>MES</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WRM OCA].
+
1WRM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WRM OCA].
==Reference==
==Reference==
-
Crystal structure of human dual specificity phosphatase, JNK stimulatory phosphatase-1, at 1.5 A resolution., Yokota T, Nara Y, Kashima A, Matsubara K, Misawa S, Kato R, Sugio S, Proteins. 2007 Feb 1;66(2):272-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17068812 17068812]
+
Crystal structure of human dual specificity phosphatase, JNK stimulatory phosphatase-1, at 1.5 A resolution., Yokota T, Nara Y, Kashima A, Matsubara K, Misawa S, Kato R, Sugio S, Proteins. 2007 Feb 1;66(2):272-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17068812 17068812]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein-tyrosine-phosphatase]]
[[Category: Protein-tyrosine-phosphatase]]
Line 23: Line 32:
[[Category: phosphatase]]
[[Category: phosphatase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:47:26 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:00:41 2008''

Revision as of 13:00, 20 March 2008

Image:1wrm.gif


PDB ID 1wrm

Drag the structure with the mouse to rotate
, resolution 1.50Å
Ligands:
Activity: Protein-tyrosine-phosphatase, with EC number 3.1.3.48
Coordinates: save as pdb, mmCIF, xml



Crystal structure of JSP-1


Overview

Human JNK stimulatory phosphatase-1 (JSP-1) is a novel member of dual specificity phosphatases. A C-terminus truncated JSP-1 was expressed in Escherichia coli and was crystallized using the sitting-drop vapor diffusion method. Thin-plate crystals obtained at 278 K belong to a monoclinic space group, C2, with unit-cell parameters a = 84.0 A, b = 49.3 A, c = 47.3 A, and beta = 119.5 degrees , and diffract up to 1.5 A resolution at 100 K. The structure of JSP-1 has a single compact (alpha/beta) domain, which consists of six alpha-helices and five beta-strands, and shows a conserved structural scaffold in regard to both DSPs and PTPs. A cleft formed by a PTP-loop at the active site is very shallow, and is occupied by one sulfonate compound, MES, at the bottom. In the binary complex structure of JSP-1 with MES, the conformations of three important segments in regard to the catalytic mechanism are not similar to those in PTP1B. JSP-1 has no loop corresponding to the Lys120-loop of PTP1B, and tryptophan residue corresponding to the substrate-stacking in PTP1B is substituted by alanine residue in JSP-1.

About this Structure

1WRM is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human dual specificity phosphatase, JNK stimulatory phosphatase-1, at 1.5 A resolution., Yokota T, Nara Y, Kashima A, Matsubara K, Misawa S, Kato R, Sugio S, Proteins. 2007 Feb 1;66(2):272-8. PMID:17068812

Page seeded by OCA on Thu Mar 20 15:00:41 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1wrm"
Personal tools