1ww1

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[[Image:1ww1.gif|left|200px]]<br /><applet load="1ww1" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1ww1.gif|left|200px]]
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caption="1ww1, resolution 2.60&Aring;" />
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'''Crystal structure of tRNase Z from Thermotoga maritima'''<br />
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{{Structure
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|PDB= 1ww1 |SIZE=350|CAPTION= <scene name='initialview01'>1ww1</scene>, resolution 2.60&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Ribonuclease_Z Ribonuclease Z], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.11 3.1.26.11]
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|GENE=
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}}
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'''Crystal structure of tRNase Z from Thermotoga maritima'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1WW1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_Z Ribonuclease Z], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.11 3.1.26.11] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WW1 OCA].
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1WW1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WW1 OCA].
==Reference==
==Reference==
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Crystal structure of the tRNA 3' processing endoribonuclease tRNase Z from Thermotoga maritima., Ishii R, Minagawa A, Takaku H, Takagi M, Nashimoto M, Yokoyama S, J Biol Chem. 2005 Apr 8;280(14):14138-44. Epub 2005 Jan 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15701599 15701599]
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Crystal structure of the tRNA 3' processing endoribonuclease tRNase Z from Thermotoga maritima., Ishii R, Minagawa A, Takaku H, Takagi M, Nashimoto M, Yokoyama S, J Biol Chem. 2005 Apr 8;280(14):14138-44. Epub 2005 Jan 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15701599 15701599]
[[Category: Ribonuclease Z]]
[[Category: Ribonuclease Z]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: riken structural genomics/proteomics initiative]]
[[Category: riken structural genomics/proteomics initiative]]
[[Category: rsgi]]
[[Category: rsgi]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:48:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:02:15 2008''

Revision as of 13:02, 20 March 2008


PDB ID 1ww1

Drag the structure with the mouse to rotate
, resolution 2.60Å
Ligands:
Activity: Ribonuclease Z, with EC number 3.1.26.11
Coordinates: save as pdb, mmCIF, xml



Crystal structure of tRNase Z from Thermotoga maritima


Overview

The maturation of the tRNA 3' end is catalyzed by a tRNA 3' processing endoribonuclease named tRNase Z (RNase Z or 3'-tRNase) in eukaryotes, Archaea, and some bacteria. The tRNase Z generally cuts the 3' extra sequence from the precursor tRNA after the discriminator nucleotide. In contrast, Thermotoga maritima tRNase Z cleaves the precursor tRNA precisely after the CCA sequence. In this study, we determined the crystal structure of T. maritima tRNase Z at 2.6-A resolution. The tRNase Z has a four-layer alphabeta/betaalpha sandwich fold, which is classified as a metallo-beta-lactamase fold, and forms a dimer. The active site is located at one edge of the beta-sandwich and is composed of conserved motifs. Based on the structure, we constructed a docking model with the tRNAs that suggests how tRNase Z may recognize the substrate tRNAs.

About this Structure

1WW1 is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.

Reference

Crystal structure of the tRNA 3' processing endoribonuclease tRNase Z from Thermotoga maritima., Ishii R, Minagawa A, Takaku H, Takagi M, Nashimoto M, Yokoyama S, J Biol Chem. 2005 Apr 8;280(14):14138-44. Epub 2005 Jan 27. PMID:15701599

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