1x8z
From Proteopedia
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| - | [[Image:1x8z.gif|left|200px]] | + | [[Image:1x8z.gif|left|200px]] |
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| - | '''Crystal structure of a pectin methylesterase inhibitor from Arabidopsis thaliana''' | + | {{Structure |
| + | |PDB= 1x8z |SIZE=350|CAPTION= <scene name='initialview01'>1x8z</scene>, resolution 2.86Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= AT1G48020 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of a pectin methylesterase inhibitor from Arabidopsis thaliana''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1X8Z is a [ | + | 1X8Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8Z OCA]. |
==Reference== | ==Reference== | ||
| - | Structural insights into the target specificity of plant invertase and pectin methylesterase inhibitory proteins., Hothorn M, Wolf S, Aloy P, Greiner S, Scheffzek K, Plant Cell. 2004 Dec;16(12):3437-47. Epub 2004 Nov 4. PMID:[http:// | + | Structural insights into the target specificity of plant invertase and pectin methylesterase inhibitory proteins., Hothorn M, Wolf S, Aloy P, Greiner S, Scheffzek K, Plant Cell. 2004 Dec;16(12):3437-47. Epub 2004 Nov 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15528298 15528298] |
[[Category: Arabidopsis thaliana]] | [[Category: Arabidopsis thaliana]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: four-helix bundle]] | [[Category: four-helix bundle]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:06:35 2008'' |
Revision as of 13:06, 20 March 2008
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| , resolution 2.86Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | AT1G48020 (Arabidopsis thaliana) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a pectin methylesterase inhibitor from Arabidopsis thaliana
Overview
Pectin methylesterase (PME) and invertase are key enzymes in plant carbohydrate metabolism. Inhibitors of both enzymes constitute a sequence family of extracellular proteins. Members of this family are selectively targeted toward either PME or invertase. In a comparative structural approach we have studied how this target specificity is implemented on homologous sequences. By extending crystallographic work on the invertase inhibitor Nt-CIF to a pectin methylesterase inhibitor (PMEI) from Arabidopsis thaliana, we show an alpha-helical hairpin motif to be an independent and mobile structural entity in PMEI. Removal of this hairpin fully inactivates the inhibitor. A chimera composed of the alpha-hairpin of PMEI and the four-helix bundle of Nt-CIF is still active against PME. By contrast, combining the corresponding segment of Nt-CIF with the four-helix bundle of PMEI renders the protein inactive toward either PME or invertase. Our experiments provide insight in how these homologous inhibitors can make differential use of similar structural modules to achieve distinct functions. Integrating our results with previous findings, we present a model for the PME-PMEI complex with important implications.
About this Structure
1X8Z is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.
Reference
Structural insights into the target specificity of plant invertase and pectin methylesterase inhibitory proteins., Hothorn M, Wolf S, Aloy P, Greiner S, Scheffzek K, Plant Cell. 2004 Dec;16(12):3437-47. Epub 2004 Nov 4. PMID:15528298
Page seeded by OCA on Thu Mar 20 15:06:35 2008
