1yg9
From Proteopedia
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| - | [[Image:1yg9.gif|left|200px]] | + | [[Image:1yg9.gif|left|200px]] |
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| - | '''The structure of mutant (N93Q) of bla g 2''' | + | {{Structure |
| + | |PDB= 1yg9 |SIZE=350|CAPTION= <scene name='initialview01'>1yg9</scene>, resolution 1.30Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene> and <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''The structure of mutant (N93Q) of bla g 2''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1YG9 is a [ | + | 1YG9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Blattella_germanica Blattella germanica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YG9 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of cockroach allergen Bla g 2, an unusual zinc binding aspartic protease with a novel mode of self-inhibition., Gustchina A, Li M, Wunschmann S, Chapman MD, Pomes A, Wlodawer A, J Mol Biol. 2005 Apr 29;348(2):433-44. PMID:[http:// | + | Crystal structure of cockroach allergen Bla g 2, an unusual zinc binding aspartic protease with a novel mode of self-inhibition., Gustchina A, Li M, Wunschmann S, Chapman MD, Pomes A, Wlodawer A, J Mol Biol. 2005 Apr 29;348(2):433-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15811379 15811379] |
[[Category: Blattella germanica]] | [[Category: Blattella germanica]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: bla g 2]] | [[Category: bla g 2]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:22:28 2008'' |
Revision as of 13:22, 20 March 2008
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| , resolution 1.30Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The structure of mutant (N93Q) of bla g 2
Overview
The crystal structure of Bla g 2 was solved in order to investigate the structural basis for the allergenic properties of this unusual protein. This is the first structure of an aspartic protease in which conserved glycine residues, in two canonical DTG triads, are substituted by different amino acid residues. Another unprecedented feature revealed by the structure is the single phenylalanine residue insertion on the tip of the flap, with the side-chain occupying the S1 binding pocket. This and other important amino acid substitutions in the active site region of Bla g 2 modify the interactions in the vicinity of the catalytic aspartate residues, increasing the distance between them to approximately 4A and establishing unique direct contacts between the flap and the catalytic residues. We attribute the absence of substantial catalytic activity in Bla g 2 to these unusual features of the active site. Five disulfide bridges and a Zn-binding site confer stability to the protein, which may contribute to sensitization at lower levels of exposure than other allergens.
About this Structure
1YG9 is a Single protein structure of sequence from Blattella germanica. Full crystallographic information is available from OCA.
Reference
Crystal structure of cockroach allergen Bla g 2, an unusual zinc binding aspartic protease with a novel mode of self-inhibition., Gustchina A, Li M, Wunschmann S, Chapman MD, Pomes A, Wlodawer A, J Mol Biol. 2005 Apr 29;348(2):433-44. PMID:15811379
Page seeded by OCA on Thu Mar 20 15:22:28 2008
Categories: Blattella germanica | Single protein | Gustchina, A. | Li, M. | Pomes, A. | Wlodawer, A. | Wuenschmann, S. | NAG | ZN | Allegren | Bla g 2
