1ytu
From Proteopedia
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| - | [[Image:1ytu.gif|left|200px]] | + | [[Image:1ytu.gif|left|200px]] |
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| - | '''Structural basis for 5'-end-specific recognition of the guide RNA strand by the A. fulgidus PIWI protein''' | + | {{Structure |
| + | |PDB= 1ytu |SIZE=350|CAPTION= <scene name='initialview01'>1ytu</scene>, resolution 2.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Structural basis for 5'-end-specific recognition of the guide RNA strand by the A. fulgidus PIWI protein''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1YTU is a [ | + | 1YTU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_dsm_4304 Archaeoglobus fulgidus dsm 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YTU OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for 5'-end-specific recognition of guide RNA by the A. fulgidus Piwi protein., Ma JB, Yuan YR, Meister G, Pei Y, Tuschl T, Patel DJ, Nature. 2005 Mar 31;434(7033):666-70. PMID:[http:// | + | Structural basis for 5'-end-specific recognition of guide RNA by the A. fulgidus Piwi protein., Ma JB, Yuan YR, Meister G, Pei Y, Tuschl T, Patel DJ, Nature. 2005 Mar 31;434(7033):666-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15800629 15800629] |
[[Category: Archaeoglobus fulgidus dsm 4304]] | [[Category: Archaeoglobus fulgidus dsm 4304]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: rna duplex]] | [[Category: rna duplex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:27:28 2008'' |
Revision as of 13:27, 20 March 2008
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Structural basis for 5'-end-specific recognition of the guide RNA strand by the A. fulgidus PIWI protein
Overview
RNA interference (RNAi) is a conserved sequence-specific gene regulatory mechanism mediated by the RNA-induced silencing complex (RISC), which is composed of a single-stranded guide RNA and an Argonaute protein. The PIWI domain, a highly conserved motif within Argonaute, has been shown to adopt an RNase H fold critical for the endonuclease cleavage activity of RISC. Here we report the crystal structure of Archaeoglobus fulgidus Piwi protein bound to double-stranded RNA, thereby identifying the binding pocket for guide-strand 5'-end recognition and providing insight into guide-strand-mediated messenger RNA target recognition. The phosphorylated 5' end of the guide RNA is anchored within a highly conserved basic pocket, supplemented by the carboxy-terminal carboxylate and a bound divalent cation. The first nucleotide from the 5' end of the guide RNA is unpaired and stacks over a conserved tyrosine residue, whereas successive nucleotides form a four-base-pair RNA duplex. Mutation of the corresponding amino acids that contact the 5' phosphate in human Ago2 resulted in attenuated mRNA cleavage activity. Our structure of the Piwi-RNA complex, and that determined elsewhere, provide direct support for the 5' region of the guide RNA serving as a nucleation site for pairing with target mRNA and for a fixed distance separating the RISC-mediated mRNA cleavage site from the anchored 5' end of the guide RNA.
About this Structure
1YTU is a Single protein structure of sequence from Archaeoglobus fulgidus dsm 4304. Full crystallographic information is available from OCA.
Reference
Structural basis for 5'-end-specific recognition of guide RNA by the A. fulgidus Piwi protein., Ma JB, Yuan YR, Meister G, Pei Y, Tuschl T, Patel DJ, Nature. 2005 Mar 31;434(7033):666-70. PMID:15800629
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