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1zm1
From Proteopedia
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| - | [[Image:1zm1.gif|left|200px]] | + | [[Image:1zm1.gif|left|200px]] |
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| - | '''Crystal structures of complex F. succinogenes 1,3-1,4-beta-D-glucanase and beta-1,3-1,4-cellotriose''' | + | {{Structure |
| + | |PDB= 1zm1 |SIZE=350|CAPTION= <scene name='initialview01'>1zm1</scene>, resolution 2.30Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Licheninase Licheninase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.73 3.2.1.73] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structures of complex F. succinogenes 1,3-1,4-beta-D-glucanase and beta-1,3-1,4-cellotriose''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ZM1 is a [ | + | 1ZM1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Fibrobacter_succinogenes Fibrobacter succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZM1 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase in complex with beta-1,3-1,4-cellotriose., Tsai LC, Shyur LF, Cheng YS, Lee SH, J Mol Biol. 2005 Dec 2;354(3):642-51. Epub 2005 Sep 30. PMID:[http:// | + | Crystal structure of truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase in complex with beta-1,3-1,4-cellotriose., Tsai LC, Shyur LF, Cheng YS, Lee SH, J Mol Biol. 2005 Dec 2;354(3):642-51. Epub 2005 Sep 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16246371 16246371] |
[[Category: Fibrobacter succinogenes]] | [[Category: Fibrobacter succinogenes]] | ||
[[Category: Licheninase]] | [[Category: Licheninase]] | ||
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[[Category: glucanase/1]] | [[Category: glucanase/1]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:37:08 2008'' |
Revision as of 13:37, 20 March 2008
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| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Licheninase, with EC number 3.2.1.73 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structures of complex F. succinogenes 1,3-1,4-beta-D-glucanase and beta-1,3-1,4-cellotriose
Overview
Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase (Fsbeta-glucanase) catalyzes the specific hydrolysis of beta-1,4 glycosidic bonds adjacent to beta-1,3 linkages in beta-D-glucans or lichenan. This is the first report to elucidate the crystal structure of a truncated Fsbeta-glucanase (TFsbeta-glucanase) in complex with beta-1,3-1,4-cellotriose, a major product of the enzyme reaction. The crystal structures, at a resolution of 2.3 angstroms, reveal that the overall fold of TFsbeta-glucanase remains virtually unchanged upon sugar binding. The enzyme accommodates five glucose residues, forming a concave active cleft. The beta-1,3-1,4-cellotriose with subsites -3 to -1 bound to the active cleft of TFsbeta-glucanase with its reducing end subsite -1 close to the key catalytic residues Glu56 and Glu60. All three subsites of the beta-1,3-1,4-cellotriose adopted a relaxed C(1)4 conformation, with a beta-1,3 glycosidic linkage between subsites -2 and -1, and a beta-1,4 glycosidic linkage between subsites -3 and -2. On the basis of the enzyme-product complex structure observed in this study, a catalytic mechanism and substrate binding conformation of the active site of TFsbeta-glucanase is proposed.
About this Structure
1ZM1 is a Single protein structure of sequence from Fibrobacter succinogenes. Full crystallographic information is available from OCA.
Reference
Crystal structure of truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase in complex with beta-1,3-1,4-cellotriose., Tsai LC, Shyur LF, Cheng YS, Lee SH, J Mol Biol. 2005 Dec 2;354(3):642-51. Epub 2005 Sep 30. PMID:16246371
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