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1zm8

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[[Image:1zm8.gif|left|200px]]<br /><applet load="1zm8" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1zm8.gif|left|200px]]
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caption="1zm8, resolution 1.90&Aring;" />
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'''Apo Crystal structure of Nuclease A from Anabaena sp.'''<br />
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{{Structure
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|PDB= 1zm8 |SIZE=350|CAPTION= <scene name='initialview01'>1zm8</scene>, resolution 1.90&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=NA:SODIUM ION'>NA</scene>
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|ACTIVITY=
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|GENE= nucA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1167 Anabaena sp.])
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}}
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'''Apo Crystal structure of Nuclease A from Anabaena sp.'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1ZM8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZM8 OCA].
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1ZM8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZM8 OCA].
==Reference==
==Reference==
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Structural insights into the mechanism of nuclease A, a betabeta alpha metal nuclease from Anabaena., Ghosh M, Meiss G, Pingoud A, London RE, Pedersen LC, J Biol Chem. 2005 Jul 29;280(30):27990-7. Epub 2005 May 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15897201 15897201]
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Structural insights into the mechanism of nuclease A, a betabeta alpha metal nuclease from Anabaena., Ghosh M, Meiss G, Pingoud A, London RE, Pedersen LC, J Biol Chem. 2005 Jul 29;280(30):27990-7. Epub 2005 May 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15897201 15897201]
[[Category: Anabaena sp.]]
[[Category: Anabaena sp.]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: nuclease]]
[[Category: nuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:16:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:37:14 2008''

Revision as of 13:37, 20 March 2008

Image:1zm8.gif


PDB ID 1zm8

Drag the structure with the mouse to rotate
, resolution 1.90Å
Ligands: , and
Gene: nucA (Anabaena sp.)
Coordinates: save as pdb, mmCIF, xml



Apo Crystal structure of Nuclease A from Anabaena sp.


Overview

Nuclease A (NucA) is a nonspecific endonuclease from Anabaena sp. capable of degrading single- and double-stranded DNA and RNA in the presence of divalent metal ions. We have determined the structure of the delta(2-24),D121A mutant of NucA in the presence of Zn2+ and Mn2+ (PDB code 1ZM8). The mutations were introduced to remove the N-terminal signal peptide and to reduce the activity of the nonspecific nuclease, thereby reducing its toxicity to the Escherichia coli expression system. NucA contains a betabeta alpha metal finger motif and a hydrated Mn2+ ion at the active site. Unexpectedly, NucA was found to contain additional metal binding sites approximately 26 A apart from the catalytic metal binding site. A structural comparison between NucA and the closest analog for which structural data exist, the Serratia nuclease, indicates several interesting differences. First, NucA is a monomer rather than a dimer. Second, there is an unexpected structural homology between the N-terminal segments despite a poorly conserved sequence, which in Serratia includes a cysteine bridge thought to play a regulatory role. In addition, although a sequence alignment had suggested that NucA lacks a proposed catalytic residue corresponding to Arg57 in Serratia, the structure determined here indicates that Arg93 in NucA is positioned to fulfill this role. Based on comparison with DNA-bound nuclease structures of the betabeta alpha metal finger nuclease family and available mutational data on NucA, we propose that His124 acts as a catalytic base, and Arg93 participates in the catalysis possibly through stabilization of the transition state.

About this Structure

1ZM8 is a Single protein structure of sequence from Anabaena sp.. Full crystallographic information is available from OCA.

Reference

Structural insights into the mechanism of nuclease A, a betabeta alpha metal nuclease from Anabaena., Ghosh M, Meiss G, Pingoud A, London RE, Pedersen LC, J Biol Chem. 2005 Jul 29;280(30):27990-7. Epub 2005 May 15. PMID:15897201

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