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1zuf
From Proteopedia
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| - | [[Image:1zuf.gif|left|200px]] | + | [[Image:1zuf.gif|left|200px]] |
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| - | '''Solution Structure of DLP-4''' | + | {{Structure |
| + | |PDB= 1zuf |SIZE=350|CAPTION= <scene name='initialview01'>1zuf</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Solution Structure of DLP-4''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ZUF is a [ | + | 1ZUF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZUF OCA]. |
==Reference== | ==Reference== | ||
| - | D-amino acid residue in a defensin-like peptide from platypus venom: effect on structure and chromatographic properties., Torres AM, Tsampazi C, Geraghty DP, Bansal PS, Alewood PF, Kuchel PW, Biochem J. 2005 Oct 15;391(Pt 2):215-20. PMID:[http:// | + | D-amino acid residue in a defensin-like peptide from platypus venom: effect on structure and chromatographic properties., Torres AM, Tsampazi C, Geraghty DP, Bansal PS, Alewood PF, Kuchel PW, Biochem J. 2005 Oct 15;391(Pt 2):215-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16033333 16033333] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Alewood, P F.]] | [[Category: Alewood, P F.]] | ||
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[[Category: helix]] | [[Category: helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:39:56 2008'' |
Revision as of 13:39, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of DLP-4
Overview
The recent discovery that the natriuretic peptide OvCNPb (Ornithorhynchus venom C-type natriuretic peptide B) from platypus (Ornithorynchus anatinus) venom contains a D-amino acid residue suggested that other D-amino-acid-containing peptides might be present in the venom. In the present study, we show that DLP-2 (defensin-like peptide-2), a 42-amino-acid residue polypeptide in the platypus venom, also contains a D-amino acid residue, D-methionine, at position 2, while DLP-4, which has an identical amino acid sequence, has all amino acids in the L-form. These findings were supported further by the detection of isomerase activity in the platypus gland venom extract that converts DLP-4 into DLP-2. In the light of this new information, the tertiary structure of DLP-2 was recalculated using a new structural template with D-Met2. The structure of DLP-4 was also determined in order to evaluate the effect of a D-amino acid at position 2 on the structure and possibly to explain the large retention time difference observed for the two molecules in reverse-phase HPLC. The solution structures of the DLP-2 and DLP-4 are very similar to each other and to the earlier reported structure of DLP-2, which assumed that all amino acids were in the L-form. Our results suggest that the incorporation of the D-amino acid at position 2 has minimal effect on the overall fold in solution.
About this Structure
1ZUF is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
D-amino acid residue in a defensin-like peptide from platypus venom: effect on structure and chromatographic properties., Torres AM, Tsampazi C, Geraghty DP, Bansal PS, Alewood PF, Kuchel PW, Biochem J. 2005 Oct 15;391(Pt 2):215-20. PMID:16033333
Page seeded by OCA on Thu Mar 20 15:39:56 2008
