2a8f
From Proteopedia
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| - | [[Image:2a8f.gif|left|200px]] | + | [[Image:2a8f.gif|left|200px]] |
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| - | '''beta-cinnamomin after sterol removal''' | + | {{Structure |
| + | |PDB= 2a8f |SIZE=350|CAPTION= <scene name='initialview01'>2a8f</scene>, resolution 1.35Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= CIN1B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4785 Phytophthora cinnamomi]) | ||
| + | }} | ||
| + | |||
| + | '''beta-cinnamomin after sterol removal''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2A8F is a [ | + | 2A8F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Phytophthora_cinnamomi Phytophthora cinnamomi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8F OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structures of the free and sterol-bound forms of beta-cinnamomin., Rodrigues ML, Archer M, Martel P, Miranda S, Thomaz M, Enguita FJ, Baptista RP, Pinho e Melo E, Sousa N, Cravador A, Carrondo MA, Biochim Biophys Acta. 2006 Jan;1764(1):110-21. Epub 2005 Oct 6. PMID:[http:// | + | Crystal structures of the free and sterol-bound forms of beta-cinnamomin., Rodrigues ML, Archer M, Martel P, Miranda S, Thomaz M, Enguita FJ, Baptista RP, Pinho e Melo E, Sousa N, Cravador A, Carrondo MA, Biochim Biophys Acta. 2006 Jan;1764(1):110-21. Epub 2005 Oct 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16249127 16249127] |
[[Category: Phytophthora cinnamomi]] | [[Category: Phytophthora cinnamomi]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sterol carrier protein]] | [[Category: sterol carrier protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:46:37 2008'' |
Revision as of 13:46, 20 March 2008
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| , resolution 1.35Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | CIN1B (Phytophthora cinnamomi) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
beta-cinnamomin after sterol removal
Overview
The crystal structure of the elicitin beta-cinnamomin (beta-CIN) was determined in complex with ergosterol at 1.1 A resolution. beta-CIN/ergosterol complex crystallized in the monoclinic space group P2(1), with unit cell parameters of a = 31.0, b = 62.8, c = 50.0 A and beta = 93.4 degrees and two molecules in the asymmetric unit. Ligand extraction with chloroform followed by crystallographic analysis yielded a 1.35 A structure of beta-CIN (P4(3)2(1)2 space group) where the characteristic elicitin fold was kept. After incubation with cholesterol, a new complex structure was obtained, showing that the protein retains, after the extraction procedure, its ability to complex sterols. The necrotic effect of beta-CIN on tobacco was also shown to remain unchanged. Theoretical docking studies of the triterpene lupeol to beta-CIN provided an explanation for the apparent inability of beta-CIN to bind this ligand, as observed experimentally.
About this Structure
2A8F is a Single protein structure of sequence from Phytophthora cinnamomi. Full crystallographic information is available from OCA.
Reference
Crystal structures of the free and sterol-bound forms of beta-cinnamomin., Rodrigues ML, Archer M, Martel P, Miranda S, Thomaz M, Enguita FJ, Baptista RP, Pinho e Melo E, Sousa N, Cravador A, Carrondo MA, Biochim Biophys Acta. 2006 Jan;1764(1):110-21. Epub 2005 Oct 6. PMID:16249127
Page seeded by OCA on Thu Mar 20 15:46:37 2008
