2asq
From Proteopedia
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| - | [[Image:2asq.gif|left|200px]] | + | [[Image:2asq.gif|left|200px]] |
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| - | '''Solution Structure of SUMO-1 in Complex with a SUMO-binding Motif (SBM)''' | + | {{Structure |
| + | |PDB= 2asq |SIZE=350|CAPTION= <scene name='initialview01'>2asq</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= SUMO1, SMT3C, SMT3H3, UBL1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), PIAS2, PIASX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Solution Structure of SUMO-1 in Complex with a SUMO-binding Motif (SBM)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2ASQ is a [ | + | 2ASQ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ASQ OCA]. |
==Reference== | ==Reference== | ||
| - | Small ubiquitin-like modifier (SUMO) recognition of a SUMO binding motif: a reversal of the bound orientation., Song J, Zhang Z, Hu W, Chen Y, J Biol Chem. 2005 Dec 2;280(48):40122-9. Epub 2005 Oct 3. PMID:[http:// | + | Small ubiquitin-like modifier (SUMO) recognition of a SUMO binding motif: a reversal of the bound orientation., Song J, Zhang Z, Hu W, Chen Y, J Biol Chem. 2005 Dec 2;280(48):40122-9. Epub 2005 Oct 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16204249 16204249] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: sumo-binding motif]] | [[Category: sumo-binding motif]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:53:25 2008'' |
Revision as of 13:53, 20 March 2008
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| Gene: | SUMO1, SMT3C, SMT3H3, UBL1 (Homo sapiens), PIAS2, PIASX (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of SUMO-1 in Complex with a SUMO-binding Motif (SBM)
Contents |
Overview
Sumoylation has recently been identified as an important mechanism that regulates protein interactions and localization in essential cellular functions, such as gene transcription, subnuclear structure formation, viral infection, and cell cycle progression. A SUMO binding amino acid sequence motif (SBM), which recognizes the SUMO moiety of modified proteins in sumoylation-dependent cellular functions, has been consistently identified by several recent studies. To understand the mechanism of SUMO recognition by the SBM, we have solved the solution structure of SUMO-1 in complex with a peptide containing the SBM derived from the protein PIASX (KVDVIDLTIESSSDEEEDPPAKR). Surprisingly, the structure reveals that the bound orientation of the SBM can reverse depending on the sequence context. The structure also reveals a novel mechanism of recognizing target sequences by a ubiquitin-like module. Unlike ubiquitin binding motifs, which all form helices and bind to the main beta-sheet of ubiquitin, the SBM forms an extended structure that binds between the alpha-helix and a beta-strand of SUMO-1. This study provides a clear mechanism of the SBM sequence variations and its recognition of the SUMO moiety in sumoylated proteins.
Disease
Known diseases associated with this structure: Orofacial cleft 10 OMIM:[601912]
About this Structure
2ASQ is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Small ubiquitin-like modifier (SUMO) recognition of a SUMO binding motif: a reversal of the bound orientation., Song J, Zhang Z, Hu W, Chen Y, J Biol Chem. 2005 Dec 2;280(48):40122-9. Epub 2005 Oct 3. PMID:16204249
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