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2axe

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[[Image:2axe.gif|left|200px]]<br /><applet load="2axe" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2axe.gif|left|200px]]
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caption="2axe, resolution 1.80&Aring;" />
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'''IODINATED COMPLEX OF ACETYL XYLAN ESTERASE AT 1.80 ANGSTROMS'''<br />
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{{Structure
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|PDB= 2axe |SIZE=350|CAPTION= <scene name='initialview01'>2axe</scene>, resolution 1.80&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Acetylesterase Acetylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.6 3.1.1.6]
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|GENE=
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}}
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'''IODINATED COMPLEX OF ACETYL XYLAN ESTERASE AT 1.80 ANGSTROMS'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2AXE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Penicillium_purpurogenum Penicillium purpurogenum] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Acetylesterase Acetylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.6 3.1.1.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AXE OCA].
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2AXE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Penicillium_purpurogenum Penicillium purpurogenum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AXE OCA].
==Reference==
==Reference==
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Determination of a protein structure by iodination: the structure of iodinated acetylxylan esterase., Ghosh D, Erman M, Sawicki M, Lala P, Weeks DR, Li N, Pangborn W, Thiel DJ, Jornvall H, Gutierrez R, Eyzaguirre J, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):779-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10089308 10089308]
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Determination of a protein structure by iodination: the structure of iodinated acetylxylan esterase., Ghosh D, Erman M, Sawicki M, Lala P, Weeks DR, Li N, Pangborn W, Thiel DJ, Jornvall H, Gutierrez R, Eyzaguirre J, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):779-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10089308 10089308]
[[Category: Acetylesterase]]
[[Category: Acetylesterase]]
[[Category: Penicillium purpurogenum]]
[[Category: Penicillium purpurogenum]]
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[[Category: esterase]]
[[Category: esterase]]
[[Category: hydrolase]]
[[Category: hydrolase]]
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[[Category: iodotyrosines]]
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[[Category: iodotyrosine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:32:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:54:59 2008''

Revision as of 13:55, 20 March 2008

Image:2axe.gif


PDB ID 2axe

Drag the structure with the mouse to rotate
, resolution 1.80Å
Ligands:
Activity: Acetylesterase, with EC number 3.1.1.6
Coordinates: save as pdb, mmCIF, xml



IODINATED COMPLEX OF ACETYL XYLAN ESTERASE AT 1.80 ANGSTROMS


Overview

Enzymatic and non-enzymatic iodination of the amino acid tyrosine is a well known phenomenon. The iodination technique has been widely used for labeling proteins. Using high-resolution X-ray crystallographic techniques, the chemical and three-dimensional structures of iodotyrosines formed by non-enzymatic incorporation of I atoms into tyrosine residues of a crystalline protein are described. Acetylxylan esterase (AXE II; 207 amino-acid residues) from Penicillium purpurogenum has substrate specificities towards acetate esters of D-xylopyranose residues in xylan and belongs to a new class of alpha/beta hydrolases. The crystals of the enzyme are highly ordered, tightly packed and diffract to better than sub-angstrom resolution at 85 K. The iodination technique has been utilized to prepare an isomorphous derivative of the AXE II crystal. The structure of the enzyme determined at 1.10 A resolution exclusively by normal and anomalous scattering from I atoms, along with the structure of the iodinated complex at 1.80 A resolution, demonstrate the formation of covalent bonds between I atoms and C atoms at ortho positions to the hydroxyl groups of two tyrosyl moieties, yielding iodotyrosines.

About this Structure

2AXE is a Single protein structure of sequence from Penicillium purpurogenum. Full crystallographic information is available from OCA.

Reference

Determination of a protein structure by iodination: the structure of iodinated acetylxylan esterase., Ghosh D, Erman M, Sawicki M, Lala P, Weeks DR, Li N, Pangborn W, Thiel DJ, Jornvall H, Gutierrez R, Eyzaguirre J, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):779-84. PMID:10089308

Page seeded by OCA on Thu Mar 20 15:54:59 2008

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