2bc3

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2bc3.gif|left|200px]]<br /><applet load="2bc3" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:2bc3.gif|left|200px]]
-
caption="2bc3, resolution 1.54&Aring;" />
+
 
-
'''T7-tagged full-length streptavidin'''<br />
+
{{Structure
 +
|PDB= 2bc3 |SIZE=350|CAPTION= <scene name='initialview01'>2bc3</scene>, resolution 1.54&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
 +
|ACTIVITY=
 +
|GENE=
 +
}}
 +
 
 +
'''T7-tagged full-length streptavidin'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
2BC3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BC3 OCA].
+
2BC3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BC3 OCA].
==Reference==
==Reference==
-
Crystallographic analysis of a full-length streptavidin with its C-terminal polypeptide bound in the biotin binding site., Le Trong I, Humbert N, Ward TR, Stenkamp RE, J Mol Biol. 2006 Feb 24;356(3):738-45. Epub 2005 Dec 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16384581 16384581]
+
Crystallographic analysis of a full-length streptavidin with its C-terminal polypeptide bound in the biotin binding site., Le Trong I, Humbert N, Ward TR, Stenkamp RE, J Mol Biol. 2006 Feb 24;356(3):738-45. Epub 2005 Dec 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16384581 16384581]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Streptomyces avidinii]]
[[Category: Streptomyces avidinii]]
Line 22: Line 31:
[[Category: t7 tag]]
[[Category: t7 tag]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:36:18 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:59:58 2008''

Revision as of 13:59, 20 March 2008

Image:2bc3.gif


PDB ID 2bc3

Drag the structure with the mouse to rotate
, resolution 1.54Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



T7-tagged full-length streptavidin


Overview

The structure of a full-length streptavidin has been determined at 1.7 A resolution and shows that the 20 residue extension at the C terminus forms a well-ordered polypeptide loop on the surface of the tetramer. Residues 150-153 of the extension are bound to the ligand-binding site, possibly competing with exogenous ligands. The binding mode of these residues is compared with that of biotin and peptidic ligands. The observed structure helps to rationalize the observations that full-length mature streptavidin binds biotinylated macromolecules with reduced affinity.

About this Structure

2BC3 is a Single protein structure of sequence from Streptomyces avidinii. Full crystallographic information is available from OCA.

Reference

Crystallographic analysis of a full-length streptavidin with its C-terminal polypeptide bound in the biotin binding site., Le Trong I, Humbert N, Ward TR, Stenkamp RE, J Mol Biol. 2006 Feb 24;356(3):738-45. Epub 2005 Dec 15. PMID:16384581

Page seeded by OCA on Thu Mar 20 15:59:58 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bc3"
Personal tools