2bdz

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[[Image:2bdz.jpg|left|200px]]<br /><applet load="2bdz" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2bdz.jpg|left|200px]]
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caption="2bdz, resolution 2.100&Aring;" />
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'''Mexicain from Jacaratia mexicana'''<br />
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{{Structure
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|PDB= 2bdz |SIZE=350|CAPTION= <scene name='initialview01'>2bdz</scene>, resolution 2.100&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=E64:N-[N-[1-HYDROXYCARBOXYETHYL-CARBONYL]LEUCYLAMINO-BUTYL]-GUANIDINE'>E64</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Mexicain from Jacaratia mexicana'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2BDZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Jacaratia_mexicana Jacaratia mexicana] with <scene name='pdbligand=E64:'>E64</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BDZ OCA].
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2BDZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Jacaratia_mexicana Jacaratia mexicana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BDZ OCA].
==Reference==
==Reference==
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Purification, crystallization and preliminary X-ray analysis of mexicain., Oliver-Salvador MC, Gonzalez-Ramirez LA, Gavira JA, Soriano-Garcia M, Garcia-Ruiz JM, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2058-60. Epub 2004, Oct 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15502326 15502326]
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Purification, crystallization and preliminary X-ray analysis of mexicain., Oliver-Salvador MC, Gonzalez-Ramirez LA, Gavira JA, Soriano-Garcia M, Garcia-Ruiz JM, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2058-60. Epub 2004, Oct 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15502326 15502326]
[[Category: Jacaratia mexicana]]
[[Category: Jacaratia mexicana]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: peptidase_c1]]
[[Category: peptidase_c1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:36:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:00:39 2008''

Revision as of 14:00, 20 March 2008

Image:2bdz.jpg


PDB ID 2bdz

Drag the structure with the mouse to rotate
, resolution 2.100Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



Mexicain from Jacaratia mexicana


Overview

Mexicain is a 23.7 kDa papain-like cysteine protease from the tropical plant Jacaratia mexicana. Extracted as a mix of proteases from the latex of the fruit, mexicain is isolated after cation-exchange chromatography as the most abundant product. The purified product inhibited with E-64 was crystallized by sitting-drop vapour diffusion in the presence of ethanolamine. Cryoprotected crystals diffracted X-rays from a home source to 1.98 A and belong to the monoclinic space group P2(1), with unit-cell parameters a = 57.36, b = 90.45, c = 80.39 A, beta = 92.64 degrees . The asymmetric unit contains four molecules of mexicain, with a corresponding crystal volume per protein weight (V(M)) of 2.24 A(3) Da(-1) and a solvent content of 45% by volume. A molecular-replacement model has been determined and refinement is in progress.

About this Structure

2BDZ is a Single protein structure of sequence from Jacaratia mexicana. Full crystallographic information is available from OCA.

Reference

Purification, crystallization and preliminary X-ray analysis of mexicain., Oliver-Salvador MC, Gonzalez-Ramirez LA, Gavira JA, Soriano-Garcia M, Garcia-Ruiz JM, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2058-60. Epub 2004, Oct 20. PMID:15502326

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