1w3y
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Hyaluronidases are enzymes that degrade hyaluronan, an important component, of the extracellular matrix. The mammalian hyaluronidases are considered, to be involved in many (patho)physiological processes like fertilization, tumor growth, and metastasis. Bacterial hyaluronidases, also termed, hyaluronate lyases, contribute to the spreading of microorganisms in, tissues. Such roles for hyaluronidases suggest that inhibitors could be, useful pharmacological tools. Potent and selective inhibitors are not, known to date, although L-ascorbic acid has been reported to be a weak, inhibitor of Streptococcus pneumoniae hyaluronate lyase (SpnHL). The x-ray, structure of SpnHL complexed with L-ascorbic acid has been elucidated, suggesting that additional hydrophobic interactions might increase, ... | + | Hyaluronidases are enzymes that degrade hyaluronan, an important component, of the extracellular matrix. The mammalian hyaluronidases are considered, to be involved in many (patho)physiological processes like fertilization, tumor growth, and metastasis. Bacterial hyaluronidases, also termed, hyaluronate lyases, contribute to the spreading of microorganisms in, tissues. Such roles for hyaluronidases suggest that inhibitors could be, useful pharmacological tools. Potent and selective inhibitors are not, known to date, although L-ascorbic acid has been reported to be a weak, inhibitor of Streptococcus pneumoniae hyaluronate lyase (SpnHL). The x-ray, structure of SpnHL complexed with L-ascorbic acid has been elucidated, suggesting that additional hydrophobic interactions might increase, inhibitory activity. Here we show that L-ascorbic acid 6-hexadecanoate, (Vcpal) is a potent inhibitor of both streptococcal and bovine testicular, hyaluronidase (BTH). Vcpal showed strong inhibition of Streptococcus, agalactiae hyaluronate lyase with an IC(50) of 4 microM and weaker, inhibition of SpnHL and BTH with IC(50) values of 100 and 56 microM, respectively. To date, Vcpal has proved to be one of the most potent, inhibitors of hyaluronidase. We also determined the x-ray structure of the, SpnHL-Vcpal complex and confirmed the hypothesis that additional, hydrophobic interactions with Phe-343, His-399, and Thr-400 in the active, site led to increased inhibition. A homology structural model of BTH was, also generated to suggest binding modes of Vcpal to this hyaluronidase., The long alkyl chain seemed to interact with an extended, hydrophobic, channel formed by mostly conserved amino acids Ala-84, Leu-91, Tyr-93, Tyr-220, and Leu-344 in BTH. |
==About this Structure== | ==About this Structure== | ||
| - | 1W3Y is a | + | 1W3Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae] with XYL, SO4 and PVC as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Hyaluronate_lyase Hyaluronate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.1 4.2.2.1] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W3Y OCA]. |
==Reference== | ==Reference== | ||
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[[Category: peptidoglycan-anchor]] | [[Category: peptidoglycan-anchor]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:26:03 2007'' |
Revision as of 11:20, 5 November 2007
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CRYSTAL STRUCTURE OF S. PNEUMONIAE HYALURONATE LYASE IN COMPLEX WITH PALMITOYL-VITAMIN C
Overview
Hyaluronidases are enzymes that degrade hyaluronan, an important component, of the extracellular matrix. The mammalian hyaluronidases are considered, to be involved in many (patho)physiological processes like fertilization, tumor growth, and metastasis. Bacterial hyaluronidases, also termed, hyaluronate lyases, contribute to the spreading of microorganisms in, tissues. Such roles for hyaluronidases suggest that inhibitors could be, useful pharmacological tools. Potent and selective inhibitors are not, known to date, although L-ascorbic acid has been reported to be a weak, inhibitor of Streptococcus pneumoniae hyaluronate lyase (SpnHL). The x-ray, structure of SpnHL complexed with L-ascorbic acid has been elucidated, suggesting that additional hydrophobic interactions might increase, inhibitory activity. Here we show that L-ascorbic acid 6-hexadecanoate, (Vcpal) is a potent inhibitor of both streptococcal and bovine testicular, hyaluronidase (BTH). Vcpal showed strong inhibition of Streptococcus, agalactiae hyaluronate lyase with an IC(50) of 4 microM and weaker, inhibition of SpnHL and BTH with IC(50) values of 100 and 56 microM, respectively. To date, Vcpal has proved to be one of the most potent, inhibitors of hyaluronidase. We also determined the x-ray structure of the, SpnHL-Vcpal complex and confirmed the hypothesis that additional, hydrophobic interactions with Phe-343, His-399, and Thr-400 in the active, site led to increased inhibition. A homology structural model of BTH was, also generated to suggest binding modes of Vcpal to this hyaluronidase., The long alkyl chain seemed to interact with an extended, hydrophobic, channel formed by mostly conserved amino acids Ala-84, Leu-91, Tyr-93, Tyr-220, and Leu-344 in BTH.
About this Structure
1W3Y is a Single protein structure of sequence from Streptococcus pneumoniae with XYL, SO4 and PVC as ligands. Active as Hyaluronate lyase, with EC number 4.2.2.1 Structure known Active Site: AC1. Full crystallographic information is available from OCA.
Reference
L-Ascorbic acid 6-hexadecanoate, a potent hyaluronidase inhibitor. X-ray structure and molecular modeling of enzyme-inhibitor complexes., Botzki A, Rigden DJ, Braun S, Nukui M, Salmen S, Hoechstetter J, Bernhardt G, Dove S, Jedrzejas MJ, Buschauer A, J Biol Chem. 2004 Oct 29;279(44):45990-7. Epub 2004 Aug 18. PMID:15322107
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