2rfk
From Proteopedia
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| - | [[ | + | ==Substrate RNA Positioning in the Archaeal H/ACA Ribonucleoprotein Complex== |
| + | <StructureSection load='2rfk' size='340' side='right' caption='[[2rfk]], [[Resolution|resolution]] 2.87Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2rfk]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RFK FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rfk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rfk RCSB], [http://www.ebi.ac.uk/pdbsum/2rfk PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/TRUB_PYRFU TRUB_PYRFU]] Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs (By similarity). [[http://www.uniprot.org/uniprot/NOP10_PYRFU NOP10_PYRFU]] Involved in ribosome biogenesis; more specifically in 18S rRNA pseudouridylation and in cleavage of pre-rRNA (By similarity). | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rf/2rfk_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The most complex RNA pseudouridylases are H/ACA ribonucleoprotein particles, which use a guide RNA for substrate capture and four proteins (Cbf5, Nop10, Gar1 and L7Ae/NHP2) for substrate modification. Here we report the three-dimensional structure of a catalytically deficient archaeal enzyme complex (including the guide RNA and three of the four essential proteins) bound to a substrate RNA. Extensive interactions of Cbf5 with one guide-substrate helix and a guide RNA stem shape the forked guide-substrate RNA complex structure and position the substrate in proximity of the Cbf5 catalytic center. Our structural and complementary fluorescence analyses also indicate that precise placement of the target uridine at the active site requires a conformation of the guide-substrate RNA duplex that is brought about by the previously identified concurrent interaction of the guide RNA with L7Ae and a composite Cbf5-Nop10 surface, and further identify a residue that is critical in this process. | ||
| - | + | Substrate RNA positioning in the archaeal H/ACA ribonucleoprotein complex.,Liang B, Xue S, Terns RM, Terns MP, Li H Nat Struct Mol Biol. 2007 Dec 2. PMID:18059286<ref>PMID:18059286</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| - | + | ==See Also== | |
| - | + | *[[TRNA pseudouridine synthase|TRNA pseudouridine synthase]] | |
| - | == | + | == References == |
| - | [[ | + | <references/> |
| - | + | __TOC__ | |
| - | == | + | </StructureSection> |
| - | < | + | |
[[Category: Pyrococcus furiosus]] | [[Category: Pyrococcus furiosus]] | ||
| - | [[Category: Li, H | + | [[Category: Li, H]] |
| - | [[Category: Liang, B | + | [[Category: Liang, B]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: Terns, M P | + | [[Category: Terns, M P]] |
| - | [[Category: Terns, R M | + | [[Category: Terns, R M]] |
| - | [[Category: Xue, S | + | [[Category: Xue, S]] |
[[Category: Archaeal h-aca ribonucleoprotein complex]] | [[Category: Archaeal h-aca ribonucleoprotein complex]] | ||
[[Category: Isomerase]] | [[Category: Isomerase]] | ||
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[[Category: Rrna processing]] | [[Category: Rrna processing]] | ||
[[Category: Secsg]] | [[Category: Secsg]] | ||
| - | [[Category: Southeast collaboratory for structural genomic]] | ||
| - | [[Category: Structural genomic]] | ||
[[Category: Trna processing]] | [[Category: Trna processing]] | ||
Revision as of 05:52, 25 December 2014
Substrate RNA Positioning in the Archaeal H/ACA Ribonucleoprotein Complex
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