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2c2i

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[[Image:2c2i.jpg|left|200px]]<br /><applet load="2c2i" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2c2i.jpg|left|200px]]
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caption="2c2i, resolution 1.80&Aring;" />
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'''STRUCTURE AND FUNCTION OF RV0130, A CONSERVED HYPOTHETICAL PROTEIN FROM M.TUBERCULOSIS'''<br />
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{{Structure
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|PDB= 2c2i |SIZE=350|CAPTION= <scene name='initialview01'>2c2i</scene>, resolution 1.80&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY= [http://en.wikipedia.org/wiki/Enoyl-CoA_hydratase Enoyl-CoA hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.17 4.2.1.17]
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|GENE=
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}}
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'''STRUCTURE AND FUNCTION OF RV0130, A CONSERVED HYPOTHETICAL PROTEIN FROM M.TUBERCULOSIS'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2C2I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Active as [http://en.wikipedia.org/wiki/Enoyl-CoA_hydratase Enoyl-CoA hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.17 4.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C2I OCA].
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2C2I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C2I OCA].
==Reference==
==Reference==
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Structure and function of Rv0130, a conserved hypothetical protein from Mycobacterium tuberculosis., Johansson P, Castell A, Jones TA, Backbro K, Protein Sci. 2006 Oct;15(10):2300-9. Epub 2006 Sep 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16963641 16963641]
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Structure and function of Rv0130, a conserved hypothetical protein from Mycobacterium tuberculosis., Johansson P, Castell A, Jones TA, Backbro K, Protein Sci. 2006 Oct;15(10):2300-9. Epub 2006 Sep 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16963641 16963641]
[[Category: Enoyl-CoA hydratase]]
[[Category: Enoyl-CoA hydratase]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: rv0130]]
[[Category: rv0130]]
[[Category: spine]]
[[Category: spine]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
[[Category: structural proteomics in europe]]
[[Category: structural proteomics in europe]]
[[Category: tuberculosis]]
[[Category: tuberculosis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:44:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:09:57 2008''

Revision as of 14:10, 20 March 2008

Image:2c2i.jpg


PDB ID 2c2i

Drag the structure with the mouse to rotate
, resolution 1.80Å
Activity: Enoyl-CoA hydratase, with EC number 4.2.1.17
Coordinates: save as pdb, mmCIF, xml



STRUCTURE AND FUNCTION OF RV0130, A CONSERVED HYPOTHETICAL PROTEIN FROM M.TUBERCULOSIS


Overview

A large fraction of the Mycobacterium tuberculosis genome codes for proteins of unknown function. We here report the structure of one of these proteins, Rv0130, solved to a resolution of 1.8 a. The Rv0130 monomer features a single hotdog fold composed of a highly curved beta-sheet on top of a long and a short alpha-helix. Two monomers in turn pack to form a double-hotdog-folded homodimer, similar to a large group of enzymes that use thiol esters as substrates. Rv0130 was found to contain a highly conserved R-specific hydratase motif buried deeply between the two monomers. Our biochemical studies show that the protein is able to hydrate a short trans-2-enoyl-coenzyme A moiety with a k(cat) of 1.1 x 10(2) sec(-1). The importance of the side chains of D40 and H45 for hydratase activity is demonstrated by site-directed mutagenesis. In contrast to many hotdog-folded proteins, a proline residue distorts the central helix of Rv0130. This distortion allows the creation of a long, curved tunnel, similar to the substrate-binding channels of long-chain eukaryotic hydratase 2 enzymes.

About this Structure

2C2I is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

Structure and function of Rv0130, a conserved hypothetical protein from Mycobacterium tuberculosis., Johansson P, Castell A, Jones TA, Backbro K, Protein Sci. 2006 Oct;15(10):2300-9. Epub 2006 Sep 8. PMID:16963641

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