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3of1

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Revision as of 07:37, 25 December 2014

Crystal Structure of Bcy1, the Yeast Regulatory Subunit of PKA

Structural highlights

3of1 is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1rgs, 1cx4
Gene:	BCY1, REG1, SRA1, YIL033C (Saccharomyces cerevisiae)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[KAPR_YEAST] Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA), an effector of the Ras/cAMP pathway. Inhibits PKA activity in the absence of cAMP. cAMP activates PKA and promotes growth and proliferation in response to good nutrient conditions. Together with ZDS1, provides a negative feedback control on the cell wall integrity-signaling pathway by acting as a negative regulator of MAP kinase SLT2/MPK1.^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

The major cAMP receptors in eukaryotes are the regulatory (R) subunits of PKA, an allosteric enzyme conserved in fungi through mammals. While mammals have four R-subunit genes, Saccharomyces cerevisiae has only one, Bcy1. To achieve a molecular understanding of PKA activation in yeast and to explore the evolution of cyclic-nucleotide binding (CNB) domains, we solved the structure of cAMP-bound Bcy1(168-416). Surprisingly, the relative orientation of the two CNB domains in Bcy1 is very different from mammalian R-subunits. This quaternary structure is defined primarily by a fungi-specific sequence in the hinge between the alphaB/alphaC helices of the CNB-A domain. The unique interface between the two CNB domains in Bcy1 defines the allosteric mechanism for cooperative activation of PKA by cAMP. Some interface motifs are isoform-specific while others, although conserved, play surprisingly different roles in each R-subunit. Phylogenetic analysis shows that structural differences in Bcy1 are shared by fungi of the subphylum Saccharomycotina.

Structure of yeast regulatory subunit: a glimpse into the evolution of PKA signaling.,Rinaldi J, Wu J, Yang J, Ralston CY, Sankaran B, Moreno S, Taylor SS Structure. 2010 Nov 10;18(11):1471-82. PMID:21070946^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Toda T, Cameron S, Sass P, Zoller M, Scott JD, McMullen B, Hurwitz M, Krebs EG, Wigler M. Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae. Mol Cell Biol. 1987 Apr;7(4):1371-7. PMID:3037314
↑ Cannon JF, Tatchell K. Characterization of Saccharomyces cerevisiae genes encoding subunits of cyclic AMP-dependent protein kinase. Mol Cell Biol. 1987 Aug;7(8):2653-63. PMID:2823100
↑ Hixson CS, Krebs EG. Characterization of a cyclic AMP-binding protein from bakers' yeast. Identification as a regulatory subunit of cyclic AMP-dependent protein kinase. J Biol Chem. 1980 Mar 10;255(5):2137-45. PMID:6243658
↑ Griffioen G, Swinnen S, Thevelein JM. Feedback inhibition on cell wall integrity signaling by Zds1 involves Gsk3 phosphorylation of a cAMP-dependent protein kinase regulatory subunit. J Biol Chem. 2003 Jun 27;278(26):23460-71. Epub 2003 Apr 18. PMID:12704202 doi:10.1074/jbc.M210691200
↑ Rinaldi J, Wu J, Yang J, Ralston CY, Sankaran B, Moreno S, Taylor SS. Structure of yeast regulatory subunit: a glimpse into the evolution of PKA signaling. Structure. 2010 Nov 10;18(11):1471-82. PMID:21070946 doi:10.1016/j.str.2010.08.013

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3of1"

@@ Line 8: / Line 8: @@
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3of1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3of1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3of1 RCSB], [http://www.ebi.ac.uk/pdbsum/3of1 PDBsum]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/KAPR_YEAST KAPR_YEAST]] Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA), an effector of the Ras/cAMP pathway. Inhibits PKA activity in the absence of cAMP. cAMP activates PKA and promotes growth and proliferation in response to good nutrient conditions. Together with ZDS1, provides a negative feedback control on the cell wall integrity-signaling pathway by acting as a negative regulator of MAP kinase SLT2/MPK1.<ref>PMID:3037314</ref> <ref>PMID:2823100</ref> <ref>PMID:6243658</ref> <ref>PMID:12704202</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

3of1

From Proteopedia

Revision as of 07:37, 25 December 2014

Crystal Structure of Bcy1, the Yeast Regulatory Subunit of PKA

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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