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2cjy

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[[Image:2cjy.gif|left|200px]]<br /><applet load="2cjy" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2cjy.gif|left|200px]]
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caption="2cjy, resolution 1.67&Aring;" />
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'''EXTENDED SUBSTRATE RECOGNITION IN CASPASE-3 REVEALED BY HIGH RESOLUTION X-RAY STRUCTURE ANALYSIS'''<br />
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{{Structure
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|PDB= 2cjy |SIZE=350|CAPTION= <scene name='initialview01'>2cjy</scene>, resolution 1.67&Aring;
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|SITE= <scene name='pdbsite=AC1:Phq+Binding+Site+For+Chain+I'>AC1</scene>
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|LIGAND= <scene name='pdbligand=PHQ:FORMIC ACID BENZYL ESTER'>PHQ</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''EXTENDED SUBSTRATE RECOGNITION IN CASPASE-3 REVEALED BY HIGH RESOLUTION X-RAY STRUCTURE ANALYSIS'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2CJY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=PHQ:'>PHQ</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Phq+Binding+Site+For+Chain+I'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CJY OCA].
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2CJY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CJY OCA].
==Reference==
==Reference==
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Extended substrate recognition in caspase-3 revealed by high resolution X-ray structure analysis., Ganesan R, Mittl PR, Jelakovic S, Grutter MG, J Mol Biol. 2006 Jun 23;359(5):1378-88. Epub 2006 May 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16787777 16787777]
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Extended substrate recognition in caspase-3 revealed by high resolution X-ray structure analysis., Ganesan R, Mittl PR, Jelakovic S, Grutter MG, J Mol Biol. 2006 Jun 23;359(5):1378-88. Epub 2006 May 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16787777 16787777]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: zymogen]]
[[Category: zymogen]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:49:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:16:21 2008''

Revision as of 14:16, 20 March 2008

Image:2cjy.gif


PDB ID 2cjy

Drag the structure with the mouse to rotate
, resolution 1.67Å
Sites:
Ligands:
Coordinates: save as pdb, mmCIF, xml



EXTENDED SUBSTRATE RECOGNITION IN CASPASE-3 REVEALED BY HIGH RESOLUTION X-RAY STRUCTURE ANALYSIS


Overview

Caspases are cysteine proteases involved in the signalling cascades of programmed cell death in which caspase-3 plays a central role, since it propagates death signals from intrinsic and extrinsic stimuli to downstream targets. The atomic resolution (1.06 Angstroms) crystal structure of the caspase-3 DEVD-cmk complex reveals the structural basis for substrate selectivity in the S4 pocket. A low-barrier hydrogen bond is observed between the side-chains of the P4 inhibitor aspartic acid and Asp179 of the N-terminal tail of the symmetry related p12 subunit. Site-directed mutagenesis of Asp179 confirmed the significance of this residue in substrate recognition. In the 1.06 Angstroms crystal structure, a radiation damage induced rearrangement of the inhibitor methylketone moiety was observed. The carbon atom that in a substrate would represent the scissile peptide bond carbonyl carbon clearly shows a tetrahedral coordination and resembles the postulated tetrahedral intermediate of the acylation reaction.

About this Structure

2CJY is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Extended substrate recognition in caspase-3 revealed by high resolution X-ray structure analysis., Ganesan R, Mittl PR, Jelakovic S, Grutter MG, J Mol Biol. 2006 Jun 23;359(5):1378-88. Epub 2006 May 11. PMID:16787777

Page seeded by OCA on Thu Mar 20 16:16:21 2008

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