1sfr

From Proteopedia

(Difference between revisions)

Revision as of 09:45, 25 December 2014

Crystal Structure of the Mycobacterium tuberculosis Antigen 85A Protein

Structural highlights

1sfr is a 3 chain structure with sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1dqy, 1dqz, 1f0p, 1f0n, 1va5
Gene:	fbpA (Mycobacterium tuberculosis)
Resources:	FirstGlance, OCA, RCSB, PDBsum, TOPSAN

Function

[A85A_MYCTU] The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor. It has a preference for C26:0-CoA over C18:1-CoA.^[1] ^[2] ^[3] ^[4]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The maintenance of the highly hydrophobic cell wall is central to the survival of Mycobacterium tuberculosis within its host environment. The antigen 85 proteins (85A, 85B, and 85C) of M. tuberculosis help maintain the integrity of the cell wall 1) by catalyzing the transfer of mycolic acids to the cell wall arabinogalactan and 2) through the synthesis of trehalose dimycolate (cord factor). Additionally, these secreted proteins allow for rapid invasion of alveolar macrophages via direct interactions between the host immune system and the invading bacillus. Here we describe two crystal structures: the structure of antigen 85C co-crystallized with octylthioglucoside as substrate, resolved to 2.0 A, and the crystal structure of antigen 85A, which was solved at a resolution of 2.7 A. The structure of 85C with the substrate analog identifies residues directly involved in substrate binding. Elucidation of the antigen 85A structure, the last of the three antigen 85 homologs to be solved, shows that the active sites of the three antigen 85 proteins are virtually identical, indicating that these share the same substrate. However, in contrast to the high level of conservation within the substrate-binding site and the active site, surface residues disparate from the active site are quite variable, indicating that three antigen 85 enzymes are needed to evade the host immune system.

Mycobacterium tuberculosis antigen 85A and 85C structures confirm binding orientation and conserved substrate specificity.,Ronning DR, Vissa V, Besra GS, Belisle JT, Sacchettini JC J Biol Chem. 2004 Aug 27;279(35):36771-7. Epub 2004 Jun 10. PMID:15192106^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Abou-Zeid C, Ratliff TL, Wiker HG, Harboe M, Bennedsen J, Rook GA. Characterization of fibronectin-binding antigens released by Mycobacterium tuberculosis and Mycobacterium bovis BCG. Infect Immun. 1988 Dec;56(12):3046-51. PMID:3141278
↑ Puech V, Guilhot C, Perez E, Tropis M, Armitige LY, Gicquel B, Daffe M. Evidence for a partial redundancy of the fibronectin-binding proteins for the transfer of mycoloyl residues onto the cell wall arabinogalactan termini of Mycobacterium tuberculosis. Mol Microbiol. 2002 May;44(4):1109-22. PMID:12010501
↑ Belisle JT, Vissa VD, Sievert T, Takayama K, Brennan PJ, Besra GS. Role of the major antigen of Mycobacterium tuberculosis in cell wall biogenesis. Science. 1997 May 30;276(5317):1420-2. PMID:9162010
↑ Elamin AA, Stehr M, Spallek R, Rohde M, Singh M. The Mycobacterium tuberculosis Ag85A is a novel diacylglycerol acyltransferase involved in lipid body formation. Mol Microbiol. 2011 Sep;81(6):1577-92. doi: 10.1111/j.1365-2958.2011.07792.x., Epub 2011 Aug 23. PMID:21819455 doi:http://dx.doi.org/10.1111/j.1365-2958.2011.07792.x
↑ Ronning DR, Vissa V, Besra GS, Belisle JT, Sacchettini JC. Mycobacterium tuberculosis antigen 85A and 85C structures confirm binding orientation and conserved substrate specificity. J Biol Chem. 2004 Aug 27;279(35):36771-7. Epub 2004 Jun 10. PMID:15192106 doi:http://dx.doi.org/10.1074/jbc.M400811200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1sfr"

@@ Line 3: / Line 3: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[1sfr]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SFR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SFR FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1dqy|1dqy]], [[1dqz|1dqz]], [[1f0p|1f0p]], [[1f0n|1f0n]], [[1va5|1va5]]</td></tr>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1dqy|1dqy]], [[1dqz|1dqz]], [[1f0p|1f0p]], [[1f0n|1f0n]], [[1va5|1va5]]</td></tr>
-<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fbpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fbpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sfr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sfr RCSB], [http://www.ebi.ac.uk/pdbsum/1sfr PDBsum], [http://www.topsan.org/Proteins/TBSGC/1sfr TOPSAN]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sfr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sfr RCSB], [http://www.ebi.ac.uk/pdbsum/1sfr PDBsum], [http://www.topsan.org/Proteins/TBSGC/1sfr TOPSAN]</span></td></tr>
-<table>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/A85A_MYCTU A85A_MYCTU]] The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor. It has a preference for C26:0-CoA over C18:1-CoA.<ref>PMID:3141278</ref> <ref>PMID:12010501</ref> <ref>PMID:9162010</ref> <ref>PMID:21819455</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 30: / Line 32: @@
 </StructureSection>
 [[Category: Mycobacterium tuberculosis]]
-[[Category: Belisle, J T.]]
+[[Category: Belisle, J T]]
-[[Category: Besra, G S.]]
+[[Category: Besra, G S]]
-[[Category: Ronning, D R.]]
+[[Category: Ronning, D R]]
-[[Category: Sacchettini, J C.]]
+[[Category: Sacchettini, J C]]
-[[Category: TBSGC, TB Structural Genomics Consortium.]]
-[[Category: Vissa, V.]]
-[[Category: Alpha/beta hydrolase]]
-[[Category: Protein structure initiative]]
-[[Category: Psi]]
 [[Category: Structural genomic]]
-[[Category: Tb structural genomics consortium]]
+[[Category: Vissa, V]]
+[[Category: Alpha/beta hydrolase]]
+[[Category: PSI, Protein structure initiative]]
 [[Category: Tbsgc]]
 [[Category: Transferase]]

1sfr

From Proteopedia

Revision as of 09:45, 25 December 2014

Crystal Structure of the Mycobacterium tuberculosis Antigen 85A Protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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