2dnj
From Proteopedia
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| - | [[Image:2dnj.gif|left|200px]] | + | [[Image:2dnj.gif|left|200px]] |
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| - | '''DNASE I-INDUCED DNA CONFORMATION. 2 ANGSTROMS STRUCTURE OF A DNASE I-OCTAMER COMPLEX''' | + | {{Structure |
| + | |PDB= 2dnj |SIZE=350|CAPTION= <scene name='initialview01'>2dnj</scene>, resolution 2.000Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Deoxyribonuclease_I Deoxyribonuclease I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.1 3.1.21.1] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''DNASE I-INDUCED DNA CONFORMATION. 2 ANGSTROMS STRUCTURE OF A DNASE I-OCTAMER COMPLEX''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2DNJ is a [ | + | 2DNJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DNJ OCA]. |
==Reference== | ==Reference== | ||
| - | DNase I-induced DNA conformation. 2 A structure of a DNase I-octamer complex., Lahm A, Suck D, J Mol Biol. 1991 Dec 5;222(3):645-67. PMID:[http:// | + | DNase I-induced DNA conformation. 2 A structure of a DNase I-octamer complex., Lahm A, Suck D, J Mol Biol. 1991 Dec 5;222(3):645-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1748997 1748997] |
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Deoxyribonuclease I]] | [[Category: Deoxyribonuclease I]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:29:16 2008'' |
Revision as of 14:29, 20 March 2008
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| , resolution 2.000Å | |||||||
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| Ligands: | |||||||
| Activity: | Deoxyribonuclease I, with EC number 3.1.21.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
DNASE I-INDUCED DNA CONFORMATION. 2 ANGSTROMS STRUCTURE OF A DNASE I-OCTAMER COMPLEX
Overview
The structure of a complex between DNase I and d(GCGATCGC)2 has been solved by molecular replacement and refined to an R-factor of 0.174 for all data between 6 and 2 A resolution. The nicked octamer duplexes have lost a dinucleotide from the 3' ends of one strand and are hydrogen-bonded across a 2-fold axis to form a quasi-continuous double helix of 14 base-pairs. DNase I is bound in the minor groove of the B-type DNA duplex forming contacts in and along both sides of the minor groove extending over a total of six base-pairs. As a consequence of binding of DNase I to the DNA-substrate the minor groove opens by about 3 A and the duplex bends towards the major groove by about 20 degrees. Apart from these more global distortions the bound duplex also shows significant deviations in local geometry. A major cause for the observed perturbations in the DNA conformation seems to be the stacking type interaction of a tyrosine ring (Y76) with a deoxyribose. In contrast, the enzyme structure is nearly unchanged compared to free DNase I (0.49 A root-mean-square deviations for main-chain atoms) thus providing a rigid framework to which the DNA substrate has to adapt on binding. These results confirm the hypothesis that groove width and stiffness are major factors determining the global sequence dependence of the enzyme's cutting rates. The nicked octamer present in the crystals did not allow us to draw detailed conclusions about the catalytic mechanism but confirmed the location of the active site near H134 on top of the central beta-sheets. A second cut of the DNA induced by diffusion of Mn2+ into the crystals may suggest the presence of a secondary active site in DNase I.
About this Structure
2DNJ is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
DNase I-induced DNA conformation. 2 A structure of a DNase I-octamer complex., Lahm A, Suck D, J Mol Biol. 1991 Dec 5;222(3):645-67. PMID:1748997
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