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2enx
From Proteopedia
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| - | [[Image:2enx.jpg|left|200px]] | + | [[Image:2enx.jpg|left|200px]] |
| - | + | ||
| - | '''Structure of the family II inorganic pyrophosphatase from Streptococcus agalactiae at 2.8 resolution''' | + | {{Structure |
| + | |PDB= 2enx |SIZE=350|CAPTION= <scene name='initialview01'>2enx</scene>, resolution 2.80Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=2PN:IMIDODIPHOSPHORIC+ACID'>2PN</scene> and <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] | ||
| + | |GENE= ppaC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1311 Streptococcus agalactiae]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of the family II inorganic pyrophosphatase from Streptococcus agalactiae at 2.8 resolution''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2ENX is a [ | + | 2ENX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae Streptococcus agalactiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ENX OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the Streptococcus agalactiae family II inorganic pyrophosphatase at 2.80 A resolution., Rantanen MK, Lehtio L, Rajagopal L, Rubens CE, Goldman A, Acta Crystallogr D Biol Crystallogr. 2007 Jun;63(Pt 6):738-43. Epub 2007, May 15. PMID:[http:// | + | Structure of the Streptococcus agalactiae family II inorganic pyrophosphatase at 2.80 A resolution., Rantanen MK, Lehtio L, Rajagopal L, Rubens CE, Goldman A, Acta Crystallogr D Biol Crystallogr. 2007 Jun;63(Pt 6):738-43. Epub 2007, May 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17505113 17505113] |
[[Category: Inorganic diphosphatase]] | [[Category: Inorganic diphosphatase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: streptococcus agalactiae]] | [[Category: streptococcus agalactiae]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:42:26 2008'' |
Revision as of 14:42, 20 March 2008
| |||||||
| , resolution 2.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , and | ||||||
| Gene: | ppaC (Streptococcus agalactiae) | ||||||
| Activity: | Inorganic diphosphatase, with EC number 3.6.1.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the family II inorganic pyrophosphatase from Streptococcus agalactiae at 2.8 resolution
Overview
Streptococcus agalactiae, a prokaryote that causes infections in neonates and immunocompromised adults, has a serine/threonine protein kinase (STK) signalling cascade. The structure of one of the targets, a family II inorganic pyrophosphatase, has been solved by molecular replacement and refined at 2.80 A resolution to an R factor of 19.2% (R(free) = 26.7%). The two monomers in the asymmetric unit are related by a noncrystallographic twofold axis, but the biological dimer is formed by a crystallographic twofold. Each monomer contains the pyrophosphate analogue imidodiphosphate (PNP) and three metal ions per active site: two Mn(2+) ions in sites M1 and M2 and an Mg(2+) ion in site M3. The enzyme is in the closed conformation. Like other family II enzymes, the structure consists of two domains (residues 1-191 and 198-311), with the active site located between them. The conformation of Lys298 in the active site is different from those observed previously and it coordinates to the conserved DHH motif in a unique way. The structure suggests that Ser150, Ser194, Ser195 and Ser296 are the most likely targets for the Ser/Thr kinase and phosphatase because they are surface-accessible and either in the active site or in the hinge region between the two domains.
About this Structure
2ENX is a Single protein structure of sequence from Streptococcus agalactiae. Full crystallographic information is available from OCA.
Reference
Structure of the Streptococcus agalactiae family II inorganic pyrophosphatase at 2.80 A resolution., Rantanen MK, Lehtio L, Rajagopal L, Rubens CE, Goldman A, Acta Crystallogr D Biol Crystallogr. 2007 Jun;63(Pt 6):738-43. Epub 2007, May 15. PMID:17505113
Page seeded by OCA on Thu Mar 20 16:42:26 2008
Categories: Inorganic diphosphatase | Single protein | Streptococcus agalactiae | Goldman, A. | Lehtio, L. | Rajagopal, L. | Rantanen, M K. | Rubens, C E. | 2PN | MG | MN | TRP | Family ii | Inorganic | Phosphorylation | Pyrophosphatase | Signalling
