2ewh
From Proteopedia
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| - | [[Image:2ewh.gif|left|200px]] | + | [[Image:2ewh.gif|left|200px]] |
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| - | '''Carboxysome protein CsoS1A from Halothiobacillus neapolitanus''' | + | {{Structure |
| + | |PDB= 2ewh |SIZE=350|CAPTION= <scene name='initialview01'>2ewh</scene>, resolution 1.400Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene> and <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= csoS1A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=927 Halothiobacillus neapolitanus]) | ||
| + | }} | ||
| + | |||
| + | '''Carboxysome protein CsoS1A from Halothiobacillus neapolitanus''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2EWH is a [ | + | 2EWH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halothiobacillus_neapolitanus Halothiobacillus neapolitanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EWH OCA]. |
==Reference== | ==Reference== | ||
| - | Structural analysis of CsoS1A and the protein shell of the Halothiobacillus neapolitanus carboxysome., Tsai Y, Sawaya MR, Cannon GC, Cai F, Williams EB, Heinhorst S, Kerfeld CA, Yeates TO, PLoS Biol. 2007 Jun;5(6):e144. PMID:[http:// | + | Structural analysis of CsoS1A and the protein shell of the Halothiobacillus neapolitanus carboxysome., Tsai Y, Sawaya MR, Cannon GC, Cai F, Williams EB, Heinhorst S, Kerfeld CA, Yeates TO, PLoS Biol. 2007 Jun;5(6):e144. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17518518 17518518] |
[[Category: Halothiobacillus neapolitanus]] | [[Category: Halothiobacillus neapolitanus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: bacterial microcompartment domain]] | [[Category: bacterial microcompartment domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:45:18 2008'' |
Revision as of 14:45, 20 March 2008
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| , resolution 1.400Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | csoS1A (Halothiobacillus neapolitanus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Carboxysome protein CsoS1A from Halothiobacillus neapolitanus
Overview
The carboxysome is a bacterial organelle that functions to enhance the efficiency of CO2 fixation by encapsulating the enzymes ribulose bisphosphate carboxylase/oxygenase (RuBisCO) and carbonic anhydrase. The outer shell of the carboxysome is reminiscent of a viral capsid, being constructed from many copies of a few small proteins. Here we describe the structure of the shell protein CsoS1A from the chemoautotrophic bacterium Halothiobacillus neapolitanus. The CsoS1A protein forms hexameric units that pack tightly together to form a molecular layer, which is perforated by narrow pores. Sulfate ions, soaked into crystals of CsoS1A, are observed in the pores of the molecular layer, supporting the idea that the pores could be the conduit for negatively charged metabolites such as bicarbonate, which must cross the shell. The problem of diffusion across a semiporous protein shell is discussed, with the conclusion that the shell is sufficiently porous to allow adequate transport of small molecules. The molecular layer formed by CsoS1A is similar to the recently observed layers formed by cyanobacterial carboxysome shell proteins. This similarity supports the argument that the layers observed represent the natural structure of the facets of the carboxysome shell. Insights into carboxysome function are provided by comparisons of the carboxysome shell to viral capsids, and a comparison of its pores to the pores of transmembrane protein channels.
About this Structure
2EWH is a Single protein structure of sequence from Halothiobacillus neapolitanus. Full crystallographic information is available from OCA.
Reference
Structural analysis of CsoS1A and the protein shell of the Halothiobacillus neapolitanus carboxysome., Tsai Y, Sawaya MR, Cannon GC, Cai F, Williams EB, Heinhorst S, Kerfeld CA, Yeates TO, PLoS Biol. 2007 Jun;5(6):e144. PMID:17518518
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