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1gik

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Revision as of 19:37, 25 December 2014

POKEWEED ANTIVIRAL PROTEIN FROM SEEDS

Structural highlights

1gik is a 1 chain structure with sequence from Phytolacca americana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Activity:	rRNA N-glycosylase, with EC number 3.2.2.22
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[RIPS_PHYAM] Inhibits viral infection of plants, and protein synthesis in vitro.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystal structure of pokeweed antiviral protein from seeds of Phytolacca americana (PAP-S) was solved at 1.8A. PAP-S is a one-chain ribosome-inactivating protein (RIP) and distinctively contains three well-defined N-acetylglucosamines, each covalently linked to an asparagine residue at positions, 10, 44, and 255, respectively. The high-resolution structure clearly shows the three mono-sugars to have either an alpha- or a beta-conformation. Two of sugars are located on the same side of the molecule with the active pocket. Except one hydrogen bond, there are no intermolecular interactions between the polypeptide chain and the sugars. Instead the sugar conformations appear to be stabilized by intermolecular interactions. The sugar structure defined at high resolution provides a structural basis for understanding their possible biological activity. The structural comparisons of PAP-S with other PAPs reveal that the major disparity of these homologous molecules is the different charge distribution on the upper right side of the front side near the active pocket. Based on the available structure of the 50S ribosomal subunit, the possible interactions between PAPs and the ribosome are discussed.

Crystal structure of pokeweed antiviral protein with well-defined sugars from seeds at 1.8A resolution.,Zeng ZH, He XL, Li HM, Hu Z, Wang DC J Struct Biol. 2003 Feb;141(2):171-8. PMID:12615543^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zeng ZH, He XL, Li HM, Hu Z, Wang DC. Crystal structure of pokeweed antiviral protein with well-defined sugars from seeds at 1.8A resolution. J Struct Biol. 2003 Feb;141(2):171-8. PMID:12615543

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1gik"

@@ Line 7: / Line 7: @@
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gik OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gik RCSB], [http://www.ebi.ac.uk/pdbsum/1gik PDBsum]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/RIPS_PHYAM RIPS_PHYAM]] Inhibits viral infection of plants, and protein synthesis in vitro.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

1gik

From Proteopedia

Revision as of 19:37, 25 December 2014

POKEWEED ANTIVIRAL PROTEIN FROM SEEDS

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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