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2w0z

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Revision as of 19:57, 25 December 2014

GRB2 SH3C (3)

Structural highlights

2w0z is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2h46, 1qg1, 1cj1, 1bm2, 1gfd, 1fyr, 1bmb, 1jyu, 1jyq, 1gfc, 1x0n, 2aob, 1tze, 1aze, 1gri, 2vvk, 1io6, 1fhs, 1zfp, 2vwf, 1gcq, 1ghu, 2aoa, 1jyr
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[GAB2_HUMAN] Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Grb2 and Gab2 form a complex implicated in normal cell signaling and cancer development. Binding of the Grb2SH3C domain to Gab2 is essential for the interaction, but molecular details remained undefined. Using peptide arrays and isothermal titration calorimetry, two Grb2SH3C binding sites in Gab2 (Gab2a and Gab2b) were confirmed and characterized. Gab2a bears similarity to a p27Kip1 epitope that also binds Grb2SH3C. Crystal structures of both Gab2 epitopes complexed with Grb2SH3C reveal that Gab2b contains a 3(10) helix that positions the arginine and lysine of the core-binding motif RxxK in parallel orientation. In contrast, the Gab2a RxxK motif is embedded in a PPII helix with Arg and Lys in staggered orientation. A similar interaction mode is also present in a new complex of Mona/GadsSH3C with an RxxxxK epitope from the putative phosphatase HD-PTP. In summary, our study reveals interaction types of SH3 domains, highlighting their great versatility.

Distinct binding modes of two epitopes in Gab2 that interact with the SH3C domain of Grb2.,Harkiolaki M, Tsirka T, Lewitzky M, Simister PC, Joshi D, Bird LE, Jones EY, O'Reilly N, Feller SM Structure. 2009 Jun 10;17(6):809-22. PMID:19523899^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wada T, Nakashima T, Oliveira-dos-Santos AJ, Gasser J, Hara H, Schett G, Penninger JM. The molecular scaffold Gab2 is a crucial component of RANK signaling and osteoclastogenesis. Nat Med. 2005 Apr;11(4):394-9. Epub 2005 Mar 6. PMID:15750601 doi:http://dx.doi.org/10.1038/nm1203
↑ Brummer T, Larance M, Herrera Abreu MT, Lyons RJ, Timpson P, Emmerich CH, Fleuren ED, Lehrbach GM, Schramek D, Guilhaus M, James DE, Daly RJ. Phosphorylation-dependent binding of 14-3-3 terminates signalling by the Gab2 docking protein. EMBO J. 2008 Sep 3;27(17):2305-16. PMID:19172738
↑ Harkiolaki M, Tsirka T, Lewitzky M, Simister PC, Joshi D, Bird LE, Jones EY, O'Reilly N, Feller SM. Distinct binding modes of two epitopes in Gab2 that interact with the SH3C domain of Grb2. Structure. 2009 Jun 10;17(6):809-22. PMID:19523899 doi:10.1016/j.str.2009.03.017

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2w0z"

@@ Line 3: / Line 3: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2w0z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W0Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W0Z FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2h46|2h46]], [[1qg1|1qg1]], [[1cj1|1cj1]], [[1bm2|1bm2]], [[1gfd|1gfd]], [[1fyr|1fyr]], [[1bmb|1bmb]], [[1jyu|1jyu]], [[1jyq|1jyq]], [[1gfc|1gfc]], [[1x0n|1x0n]], [[2aob|2aob]], [[1tze|1tze]], [[1aze|1aze]], [[1gri|1gri]], [[2vvk|2vvk]], [[1io6|1io6]], [[1fhs|1fhs]], [[1zfp|1zfp]], [[2vwf|2vwf]], [[1gcq|1gcq]], [[1ghu|1ghu]], [[2aoa|2aoa]], [[1jyr|1jyr]]</td></tr>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2h46|2h46]], [[1qg1|1qg1]], [[1cj1|1cj1]], [[1bm2|1bm2]], [[1gfd|1gfd]], [[1fyr|1fyr]], [[1bmb|1bmb]], [[1jyu|1jyu]], [[1jyq|1jyq]], [[1gfc|1gfc]], [[1x0n|1x0n]], [[2aob|2aob]], [[1tze|1tze]], [[1aze|1aze]], [[1gri|1gri]], [[2vvk|2vvk]], [[1io6|1io6]], [[1fhs|1fhs]], [[1zfp|1zfp]], [[2vwf|2vwf]], [[1gcq|1gcq]], [[1ghu|1ghu]], [[2aoa|2aoa]], [[1jyr|1jyr]]</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w0z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w0z RCSB], [http://www.ebi.ac.uk/pdbsum/2w0z PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w0z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w0z RCSB], [http://www.ebi.ac.uk/pdbsum/2w0z PDBsum]</span></td></tr>
-<table>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/GAB2_HUMAN GAB2_HUMAN]] Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.<ref>PMID:15750601</ref> <ref>PMID:19172738</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 34: @@
 </StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Feller, S M.]]
+[[Category: Feller, S M]]
-[[Category: Harkiolaki, M.]]
+[[Category: Harkiolaki, M]]
-[[Category: Tsirka, T.]]
+[[Category: Tsirka, T]]
 [[Category: Golgi apparatus]]
 [[Category: Grb2]]

2w0z

From Proteopedia

Revision as of 19:57, 25 December 2014

GRB2 SH3C (3)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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