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3onj

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Revision as of 22:16, 25 December 2014

Crystal structure of yeast Vti1p_Habc domain

Structural highlights

3onj is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	3onk, 3onl
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[VTI1_YEAST] t-SNARE found in various SNARE complexes involved in multiple transport pathways. The composition of the t-SNARE complexes is specific for a limited number of v-SNAREs and therefore allows only the vesicles carrying the matching v-SNARE to fuse.^[1] ^[2] ^[3]

Publication Abstract from PubMed

SNARE proteins are crucial for membrane fusion in vesicular transport. To ensure efficient and accurate fusion, SNAREs need to be sorted into different budding vesicles. This process is usually regulated by specific recognition between SNAREs and their adaptor proteins. How different pairs of SNAREs and adaptors achieve their recognition is unclear. Here, we report the recognition between yeast SNARE Vti1p and its adaptor Ent3p derived from three crystal structures. Surprisingly, this yeast pair Vti1p/Ent3p interacts through a distinct binding site compared to their homologues vti1b/epsinR in mammals. An opposite surface on Vti1p_Habc domain binds to a conserved area on the epsin N-terminal homology (ENTH) domain of Ent3p. Two-hybrid, in vitro pull-down and in vivo experiments indicate this binding interface is important for correct localization of Vti1p in the cell. This previously undescribed discovery that a cargo and adaptor pair uses different binding sites across species suggests the diversity of SNARE-adaptor recognition in vesicular transport.

Epsin N-terminal homology domains bind on opposite sides of two SNAREs.,Wang J, Gossing M, Fang P, Zimmermann J, Li X, von Mollard GF, Niu L, Teng M Proc Natl Acad Sci U S A. 2011 Jul 26;108(30):12277-82. Epub 2011 Jul 11. PMID:21746902^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ungermann C, von Mollard GF, Jensen ON, Margolis N, Stevens TH, Wickner W. Three v-SNAREs and two t-SNAREs, present in a pentameric cis-SNARE complex on isolated vacuoles, are essential for homotypic fusion. J Cell Biol. 1999 Jun 28;145(7):1435-42. PMID:10385523
↑ Paumet F, Brugger B, Parlati F, McNew JA, Sollner TH, Rothman JE. A t-SNARE of the endocytic pathway must be activated for fusion. J Cell Biol. 2001 Dec 10;155(6):961-8. Epub 2001 Dec 10. PMID:11739407 doi:http://dx.doi.org/10.1083/jcb.200104092
↑ Paumet F, Rahimian V, Rothman JE. The specificity of SNARE-dependent fusion is encoded in the SNARE motif. Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3376-80. Epub 2004 Feb 23. PMID:14981247 doi:http://dx.doi.org/10.1073/pnas.0400271101
↑ Wang J, Gossing M, Fang P, Zimmermann J, Li X, von Mollard GF, Niu L, Teng M. Epsin N-terminal homology domains bind on opposite sides of two SNAREs. Proc Natl Acad Sci U S A. 2011 Jul 26;108(30):12277-82. Epub 2011 Jul 11. PMID:21746902 doi:10.1073/pnas.1013101108

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3onj"

@@ Line 3: / Line 3: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[3onj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ONJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ONJ FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3onk|3onk]], [[3onl|3onl]]</td></tr>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3onk|3onk]], [[3onl|3onl]]</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3onj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3onj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3onj RCSB], [http://www.ebi.ac.uk/pdbsum/3onj PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3onj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3onj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3onj RCSB], [http://www.ebi.ac.uk/pdbsum/3onj PDBsum]</span></td></tr>
-<table>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/VTI1_YEAST VTI1_YEAST]] t-SNARE found in various SNARE complexes involved in multiple transport pathways. The composition of the t-SNARE complexes is specific for a limited number of v-SNAREs and therefore allows only the vesicles carrying the matching v-SNARE to fuse.<ref>PMID:10385523</ref> <ref>PMID:11739407</ref> <ref>PMID:14981247</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 12: / Line 14: @@
 Epsin N-terminal homology domains bind on opposite sides of two SNAREs.,Wang J, Gossing M, Fang P, Zimmermann J, Li X, von Mollard GF, Niu L, Teng M Proc Natl Acad Sci U S A. 2011 Jul 26;108(30):12277-82. Epub 2011 Jul 11. PMID:21746902<ref>PMID:21746902</ref>
-From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
 == References ==
@@ Line 19: / Line 21: @@
 </StructureSection>
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Fang, P.]]
+[[Category: Fang, P]]
-[[Category: Niu, L.]]
+[[Category: Niu, L]]
-[[Category: Teng, M.]]
+[[Category: Teng, M]]
-[[Category: Wang, J.]]
+[[Category: Wang, J]]
 [[Category: Habc]]
 [[Category: Helix]]
 [[Category: Protein transport]]

3onj

From Proteopedia

Revision as of 22:16, 25 December 2014

Crystal structure of yeast Vti1p_Habc domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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