2g8h

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[[Image:2g8h.gif|left|200px]]<br /><applet load="2g8h" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2g8h.gif|left|200px]]
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caption="2g8h, resolution 1.85&Aring;" />
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'''B. halodurans RNase H catalytic domain D192N mutant in complex with Mg2+ and RNA/DNA hybrid (non-P nick at the active site)'''<br />
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{{Structure
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|PDB= 2g8h |SIZE=350|CAPTION= <scene name='initialview01'>2g8h</scene>, resolution 1.85&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=VO4:VANADATE ION'>VO4</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4]
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|GENE= rnhA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=86665 Bacillus halodurans])
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}}
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'''B. halodurans RNase H catalytic domain D192N mutant in complex with Mg2+ and RNA/DNA hybrid (non-P nick at the active site)'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2G8H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_halodurans Bacillus halodurans] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=VO4:'>VO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8H OCA].
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2G8H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_halodurans Bacillus halodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G8H OCA].
==Reference==
==Reference==
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Stepwise analyses of metal ions in RNase H catalysis from substrate destabilization to product release., Nowotny M, Yang W, EMBO J. 2006 May 3;25(9):1924-33. Epub 2006 Apr 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16601679 16601679]
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Stepwise analyses of metal ions in RNase H catalysis from substrate destabilization to product release., Nowotny M, Yang W, EMBO J. 2006 May 3;25(9):1924-33. Epub 2006 Apr 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16601679 16601679]
[[Category: Bacillus halodurans]]
[[Category: Bacillus halodurans]]
[[Category: Ribonuclease H]]
[[Category: Ribonuclease H]]
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[[Category: rnase h]]
[[Category: rnase h]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:29:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:01:38 2008''

Revision as of 15:01, 20 March 2008

Image:2g8h.gif


PDB ID 2g8h

Drag the structure with the mouse to rotate
, resolution 1.85Å
Ligands: and
Gene: rnhA (Bacillus halodurans)
Activity: Ribonuclease H, with EC number 3.1.26.4
Coordinates: save as pdb, mmCIF, xml



B. halodurans RNase H catalytic domain D192N mutant in complex with Mg2+ and RNA/DNA hybrid (non-P nick at the active site)


Overview

In two-metal catalysis, metal ion A has been proposed to activate the nucleophile and metal ion B to stabilize the transition state. We recently reported crystal structures of RNase H-RNA/DNA substrate complexes obtained at 1.5-2.2 Angstroms. We have now determined and report here structures of reaction intermediate and product complexes of RNase H at 1.65-1.85 Angstroms. The movement of the two metal ions suggests how they may facilitate RNA hydrolysis during the catalytic process. Firstly, metal ion A may assist nucleophilic attack by moving towards metal ion B and bringing the nucleophile close to the scissile phosphate. Secondly, metal ion B transforms from an irregular coordination in the substrate complex to a more regular geometry in the product complex. The exquisite sensitivity of Mg(2+) to the coordination environment likely destabilizes the enzyme-substrate complex and reduces the energy barrier to form product. Lastly, product release probably requires dissociation of metal ion A, which is inhibited by either high concentrations of divalent cations or mutation of an assisting protein residue.

About this Structure

2G8H is a Single protein structure of sequence from Bacillus halodurans. Full crystallographic information is available from OCA.

Reference

Stepwise analyses of metal ions in RNase H catalysis from substrate destabilization to product release., Nowotny M, Yang W, EMBO J. 2006 May 3;25(9):1924-33. Epub 2006 Apr 6. PMID:16601679

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