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2gaf
From Proteopedia
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| - | [[Image:2gaf.gif|left|200px]] | + | [[Image:2gaf.gif|left|200px]] |
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| - | '''Crystal Structure of the Vaccinia Polyadenylate Polymerase Heterodimer (apo form)''' | + | {{Structure |
| + | |PDB= 2gaf |SIZE=350|CAPTION= <scene name='initialview01'>2gaf</scene>, resolution 2.400Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/mRNA_(nucleoside-2'-O-)-methyltransferase mRNA (nucleoside-2'-O-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.57 2.1.1.57] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the Vaccinia Polyadenylate Polymerase Heterodimer (apo form)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2GAF is a [ | + | 2GAF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GAF OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structures of the vaccinia virus polyadenylate polymerase heterodimer: insights into ATP selectivity and processivity., Moure CM, Bowman BR, Gershon PD, Quiocho FA, Mol Cell. 2006 May 5;22(3):339-49. PMID:[http:// | + | Crystal structures of the vaccinia virus polyadenylate polymerase heterodimer: insights into ATP selectivity and processivity., Moure CM, Bowman BR, Gershon PD, Quiocho FA, Mol Cell. 2006 May 5;22(3):339-49. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16678106 16678106] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Vaccinia virus]] | [[Category: Vaccinia virus]] | ||
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[[Category: processivity]] | [[Category: processivity]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:02:20 2008'' |
Revision as of 15:02, 20 March 2008
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| , resolution 2.400Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | mRNA (nucleoside-2'-O-)-methyltransferase, with EC number 2.1.1.57 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Vaccinia Polyadenylate Polymerase Heterodimer (apo form)
Overview
Polyadenylation of mRNAs in poxviruses, crucial for virion maturation, is carried out by a poly(A) polymerase heterodimer composed of a catalytic component, VP55, and a processivity factor, VP39. The ATP-gamma-S bound and unbound crystal structures of the vaccinia polymerase reveal an unusual architecture for VP55 that comprises of N-terminal, central or catalytic, and C-terminal domains with different topologies and that differs from many polymerases, including the eukaryotic poly(A) polymerases. Residues in the active site of VP55, located between the catalytic and C-terminal domains, make specific interactions with the adenine of the ATP analog, establishing the molecular basis of ATP recognition. VP55's concave surface docks the globular VP39. A model for RNA primer binding that involves all three VP55 domains and VP39 is proposed. The model supports biochemical evidence that VP39 functions as a processivity factor by partially enclosing the RNA primer at the heterodimer interface.
About this Structure
2GAF is a Protein complex structure of sequences from Vaccinia virus. Full crystallographic information is available from OCA.
Reference
Crystal structures of the vaccinia virus polyadenylate polymerase heterodimer: insights into ATP selectivity and processivity., Moure CM, Bowman BR, Gershon PD, Quiocho FA, Mol Cell. 2006 May 5;22(3):339-49. PMID:16678106
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