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3zi5
From Proteopedia
(Difference between revisions)
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| - | + | ==Crystal STRUCTURE OF RESTRICTION ENDONUCLEASE BFII C-TERMINAL RECOGNITION DOMAIN IN COMPLEX WITH COGNATE DNA== | |
| - | + | <StructureSection load='3zi5' size='340' side='right' caption='[[3zi5]], [[Resolution|resolution]] 3.20Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3zi5]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14575 Atcc 14575]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZI5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZI5 FirstGlance]. <br> | ||
| + | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zi5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zi5 RCSB], [http://www.ebi.ac.uk/pdbsum/3zi5 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The B3 DNA-binding domains (DBDs) of plant transcription factors (TF) and DBDs of EcoRII and BfiI restriction endonucleases (EcoRII-N and BfiI-C) share a common structural fold, classified as the DNA-binding pseudobarrel. The B3 DBDs in the plant TFs recognize a diverse set of target sequences. The only available co-crystal structure of the B3-like DBD is that of EcoRII-N (recognition sequence 5'-CCTGG-3'). In order to understand the structural and molecular mechanisms of specificity of B3 DBDs, we have solved the crystal structure of BfiI-C (recognition sequence 5'-ACTGGG-3') complexed with 12-bp cognate oligoduplex. Structural comparison of BfiI-C-DNA and EcoRII-N-DNA complexes reveals a conserved DNA-binding mode and a conserved pattern of interactions with the phosphodiester backbone. The determinants of the target specificity are located in the loops that emanate from the conserved structural core. The BfiI-C-DNA structure presented here expands a range of templates for modeling of the DNA-bound complexes of the B3 family of plant TFs. | ||
| - | + | Structural insight into the specificity of the B3 DNA-binding domains provided by the co-crystal structure of the C-terminal fragment of BfiI restriction enzyme.,Golovenko D, Manakova E, Zakrys L, Zaremba M, Sasnauskas G, Grazulis S, Siksnys V Nucleic Acids Res. 2014 Jan 13. PMID:24423868<ref>PMID:24423868</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Atcc 14575]] | [[Category: Atcc 14575]] | ||
[[Category: Type II site-specific deoxyribonuclease]] | [[Category: Type II site-specific deoxyribonuclease]] | ||
| - | [[Category: Golovenko, D | + | [[Category: Golovenko, D]] |
| - | [[Category: Grazulis, S | + | [[Category: Grazulis, S]] |
| - | [[Category: Manakova, E | + | [[Category: Manakova, E]] |
| - | [[Category: Sasnauskas, G | + | [[Category: Sasnauskas, G]] |
| - | [[Category: Siksnys, V | + | [[Category: Siksnys, V]] |
| - | [[Category: Zakrys, L | + | [[Category: Zakrys, L]] |
| - | [[Category: Zaremba, M | + | [[Category: Zaremba, M]] |
[[Category: Dna recognition]] | [[Category: Dna recognition]] | ||
[[Category: Dna-binding pseudobarrel fold]] | [[Category: Dna-binding pseudobarrel fold]] | ||
[[Category: Hydrolase-dna complex]] | [[Category: Hydrolase-dna complex]] | ||
Revision as of 09:22, 4 January 2015
Crystal STRUCTURE OF RESTRICTION ENDONUCLEASE BFII C-TERMINAL RECOGNITION DOMAIN IN COMPLEX WITH COGNATE DNA
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