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2gsq
From Proteopedia
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| - | [[Image:2gsq.jpg|left|200px]] | + | [[Image:2gsq.jpg|left|200px]] |
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| - | '''GLUTATHIONE S-TRANSFERASE FROM SQUID DIGESTIVE GLAND COMPLEXED WITH S-(3-IODOBENZYL)GLUTATHIONE''' | + | {{Structure |
| + | |PDB= 2gsq |SIZE=350|CAPTION= <scene name='initialview01'>2gsq</scene>, resolution 2.2Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=GBI:S-(3-IODOBENZYL)GLUTATHIONE'>GBI</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] | ||
| + | |GENE= CDNA INSERT OF CLONE PGST5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6633 Ommastrephes sloani]) | ||
| + | }} | ||
| + | |||
| + | '''GLUTATHIONE S-TRANSFERASE FROM SQUID DIGESTIVE GLAND COMPLEXED WITH S-(3-IODOBENZYL)GLUTATHIONE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2GSQ is a [ | + | 2GSQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Ommastrephes_sloani Ommastrephes sloani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GSQ OCA]. |
==Reference== | ==Reference== | ||
| - | Location of a potential transport binding site in a sigma class glutathione transferase by x-ray crystallography., Ji X, von Rosenvinge EC, Johnson WW, Armstrong RN, Gilliland GL, Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8208-13. PMID:[http:// | + | Location of a potential transport binding site in a sigma class glutathione transferase by x-ray crystallography., Ji X, von Rosenvinge EC, Johnson WW, Armstrong RN, Gilliland GL, Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8208-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8710848 8710848] |
[[Category: Glutathione transferase]] | [[Category: Glutathione transferase]] | ||
[[Category: Ommastrephes sloani]] | [[Category: Ommastrephes sloani]] | ||
| Line 22: | Line 31: | ||
[[Category: squid digestive gland]] | [[Category: squid digestive gland]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:08:35 2008'' |
Revision as of 15:08, 20 March 2008
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| , resolution 2.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | CDNA INSERT OF CLONE PGST5 (Ommastrephes sloani) | ||||||
| Activity: | Glutathione transferase, with EC number 2.5.1.18 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
GLUTATHIONE S-TRANSFERASE FROM SQUID DIGESTIVE GLAND COMPLEXED WITH S-(3-IODOBENZYL)GLUTATHIONE
Overview
The crystal structure of the sigma class glutathione transferase from squid digestive gland in complex with S-(3-iodobenzyl)glutathione reveals a third binding site for the glutathione conjugate besides the two in the active sites of the dimer. The additional binding site is near the crystallographic two-fold axis between the two alpha 4-turn-alpha 5 motifs. The principal binding interactions with the conjugate include specific electrostatic interactions between the peptide and the two subunits and a hydrophobic cavity found across the two-fold axis that accommodates the 3-iodobenzyl group. Thus, two identical, symmetry-related but mutually exclusive binding modes for the third conjugate are observed. The hydrophobic pocket is about 14 A from the hydroxyl group of Tyr-7 in the active site. This site is a potential transport binding site for hydrophobic molecules or their glutathione conjugates.
About this Structure
2GSQ is a Single protein structure of sequence from Ommastrephes sloani. Full crystallographic information is available from OCA.
Reference
Location of a potential transport binding site in a sigma class glutathione transferase by x-ray crystallography., Ji X, von Rosenvinge EC, Johnson WW, Armstrong RN, Gilliland GL, Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8208-13. PMID:8710848
Page seeded by OCA on Thu Mar 20 17:08:35 2008
