This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4mum

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
{{STRUCTURE_4mum| PDB=4mum | SCENE= }}
+
==Crystal structure of mitochondrial 5'(3')-deoxy ribonucleotidase alternative spliced variant==
-
===Crystal structure of mitochondrial 5'(3')-deoxy ribonucleotidase alternative spliced variant===
+
<StructureSection load='4mum' size='340' side='right' caption='[[4mum]], [[Resolution|resolution]] 1.27&Aring;' scene=''>
-
{{ABSTRACT_PUBMED_24506201}}
+
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[4mum]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MUM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MUM FirstGlance]. <br>
 +
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
 +
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DNT1, NT5C, UMPH2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mum OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mum RCSB], [http://www.ebi.ac.uk/pdbsum/4mum PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Abstract Human mitochondrial 5'(3')-deoxyribonucleotidase (mdN) catalyzes dephosphorylation of nucleoside monophosphates, and thus helps maintain homeostasis of deoxynucleosides required for mitochondrial DNA synthesis. Mature mdN is a 23-kDa dimeric protein with highest expression levels in the heart, brain and skeletal muscle. We have identified an alternative splice variant of the mdN gene containing an 18-nucleotide insertion encoding 6 amino acids (GKWPAT) at the 3'-end of the penultimate exon 4. We recombinantly expressed this enzyme variant and characterized its biochemical and kinetic properties as well as its three-dimensional structure. Our high-resolution (1.27 A) crystal structure revealed that the insertion forms a loop located in the vicinity of the active site pocket and affects enzyme kinetic parameters as well as protein thermal stability.
-
==About this Structure==
+
Kinetic and structural characterization of an alternatively spliced variant of human mitochondrial 5'(3')-deoxyribonucleotidase.,Pachl P, Fabry M, Veverka V, Brynda J, Rezacova P J Enzyme Inhib Med Chem. 2014 Feb 10. PMID:24506201<ref>PMID:24506201</ref>
-
[[4mum]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MUM OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<ref group="xtra">PMID:024506201</ref><references group="xtra"/><references/>
+
</div>
-
[[Category: Pachl, P.]]
+
== References ==
-
[[Category: Rezacova, P.]]
+
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Human]]
 +
[[Category: Pachl, P]]
 +
[[Category: Rezacova, P]]
[[Category: 5'-nucleotidase]]
[[Category: 5'-nucleotidase]]
[[Category: Dephosphorylation]]
[[Category: Dephosphorylation]]

Revision as of 09:02, 5 January 2015

Crystal structure of mitochondrial 5'(3')-deoxy ribonucleotidase alternative spliced variant

4mum, resolution 1.27Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4mum"
Personal tools