2hhc
From Proteopedia
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| - | [[Image:2hhc.jpg|left|200px]] | + | [[Image:2hhc.jpg|left|200px]] |
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| - | '''Crystal structure of fucosyltransferase NodZ from Bradyrhizobium''' | + | {{Structure |
| + | |PDB= 2hhc |SIZE=350|CAPTION= <scene name='initialview01'>2hhc</scene>, resolution 1.54Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Po4+Binding+Site+For+Residue+A+1001'>AC1</scene>, <scene name='pdbsite=AC2:Po4+Binding+Site+For+Residue+A+1002'>AC2</scene>, <scene name='pdbsite=AC3:Po4+Binding+Site+For+Residue+A+1003'>AC3</scene> and <scene name='pdbsite=AC4:Trs+Binding+Site+For+Residue+A+2001'>AC4</scene> | ||
| + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= nodZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=376 Bradyrhizobium sp.]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of fucosyltransferase NodZ from Bradyrhizobium''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HHC is a [ | + | 2HHC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_sp. Bradyrhizobium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HHC OCA]. |
==Reference== | ==Reference== | ||
| - | High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor., Brzezinski K, Stepkowski T, Panjikar S, Bujacz G, Jaskolski M, Acta Biochim Pol. 2007;54(3):537-49. Epub 2007 Aug 30. PMID:[http:// | + | High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor., Brzezinski K, Stepkowski T, Panjikar S, Bujacz G, Jaskolski M, Acta Biochim Pol. 2007;54(3):537-49. Epub 2007 Aug 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17762900 17762900] |
[[Category: Bradyrhizobium sp.]] | [[Category: Bradyrhizobium sp.]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: nodz]] | [[Category: nodz]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:17:07 2008'' |
Revision as of 15:17, 20 March 2008
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| , resolution 1.54Å | |||||||
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| Sites: | , , and | ||||||
| Ligands: | and | ||||||
| Gene: | nodZ (Bradyrhizobium sp.) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of fucosyltransferase NodZ from Bradyrhizobium
Overview
The fucosyltransferase NodZ is involved in the biosynthesis of the nodulation factor in nitrogen-fixing symbiotic bacteria. It catalyzes alpha1,6 transfer of l-fucose from GDP-fucose to the reducing residue of the synthesized Nod oligosaccharide. We present the structure of the NodZ protein from Bradyrhizobium expressed in Escherichia coli and crystallized in the presence of phosphate ions in two crystal forms. The enzyme is arranged into two domains of nearly equal size. Although NodZ falls in one broad class (GT-B) with other two-domain glycosyltransferases, the topology of its domains deviates from the canonical Rossmann fold, with particularly high distortions in the N-terminal domain. Mutational data combined with structural and sequence alignments indicate residues of potential importance in GDP-fucose binding or in the catalytic mechanism. They are all clustered in three conserved sequence motifs located in the C-terminal domain.
About this Structure
2HHC is a Single protein structure of sequence from Bradyrhizobium sp.. Full crystallographic information is available from OCA.
Reference
High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor., Brzezinski K, Stepkowski T, Panjikar S, Bujacz G, Jaskolski M, Acta Biochim Pol. 2007;54(3):537-49. Epub 2007 Aug 30. PMID:17762900
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