2hp4

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[[Image:2hp4.jpg|left|200px]]<br /><applet load="2hp4" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2hp4.jpg|left|200px]]
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caption="2hp4, resolution 2.10&Aring;" />
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'''Computational design and crystal structure of an enhanced affinity mutant human CD8-alpha-alpha co-receptor'''<br />
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{{Structure
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|PDB= 2hp4 |SIZE=350|CAPTION= <scene name='initialview01'>2hp4</scene>, resolution 2.10&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Computational design and crystal structure of an enhanced affinity mutant human CD8-alpha-alpha co-receptor'''
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==Overview==
==Overview==
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==Disease==
==Disease==
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Known diseases associated with this structure: CD8 deficiency, familial OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=186910 186910]], Megakaryoblastic leukemia, acute OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=606078 606078]]
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Known disease associated with this structure: Megakaryoblastic leukemia, acute OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=606078 606078]]
==About this Structure==
==About this Structure==
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2HP4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP4 OCA].
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2HP4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP4 OCA].
==Reference==
==Reference==
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Computational design and crystal structure of an enhanced affinity mutant human CD8 alphaalpha coreceptor., Cole DK, Rizkallah PJ, Boulter JM, Sami M, Vuidepot AL, Glick M, Gao F, Bell JI, Jakobsen BK, Gao GF, Proteins. 2007 Apr 1;67(1):65-74. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17243170 17243170]
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Computational design and crystal structure of an enhanced affinity mutant human CD8 alphaalpha coreceptor., Cole DK, Rizkallah PJ, Boulter JM, Sami M, Vuidepot AL, Glick M, Gao F, Bell JI, Jakobsen BK, Gao GF, Proteins. 2007 Apr 1;67(1):65-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17243170 17243170]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: soluble protein]]
[[Category: soluble protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:44:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:19:44 2008''

Revision as of 15:19, 20 March 2008

Image:2hp4.jpg


PDB ID 2hp4

Drag the structure with the mouse to rotate
, resolution 2.10Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



Computational design and crystal structure of an enhanced affinity mutant human CD8-alpha-alpha co-receptor


Contents

Overview

Human CD8 is a T cell coreceptor, which binds to pHLA I and plays a pivotal role in the activation of cytotoxic T lymphocytes. Soluble recombinant CD8 alphaalpha has been shown to antagonize T cell activation, both in vitro and in vivo. However, because of a very low affinity for pHLA I, high concentrations of soluble CD8 alphaalpha are required for efficient inhibition. Based upon our knowledge of the wild-type CD8/pHLA I structure, we have designed and produced a mutated form of soluble CD8 alphaalpha that binds to pHLA I with approximately fourfold higher affinity. We have characterized the binding of the high affinity CD8 mutant using surface plasmon resonance and determined its structure at 2.1 A resolution using X-ray crystallography. The analysis of this structure suggests that the higher affinity is achieved by providing a larger side chain that allows for an optimal contact to be made between the HLA alpha3 loop and the mutated CDR-like loops of CD8.

Disease

Known disease associated with this structure: Megakaryoblastic leukemia, acute OMIM:[606078]

About this Structure

2HP4 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Computational design and crystal structure of an enhanced affinity mutant human CD8 alphaalpha coreceptor., Cole DK, Rizkallah PJ, Boulter JM, Sami M, Vuidepot AL, Glick M, Gao F, Bell JI, Jakobsen BK, Gao GF, Proteins. 2007 Apr 1;67(1):65-74. PMID:17243170

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