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2id8

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[[Image:2id8.gif|left|200px]]<br /><applet load="2id8" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2id8.gif|left|200px]]
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caption="2id8, resolution 1.27&Aring;" />
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'''Crystal structure of Proteinase K'''<br />
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{{Structure
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|PDB= 2id8 |SIZE=350|CAPTION= <scene name='initialview01'>2id8</scene>, resolution 1.27&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=2DB:(S)-(2,3-DIHYDROXYPROPOXY)TRIHYDROXYBORATE'>2DB</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64]
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|GENE=
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}}
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'''Crystal structure of Proteinase K'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2ID8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Engyodontium_album Engyodontium album] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=NO3:'>NO3</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=2DB:'>2DB</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ID8 OCA].
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2ID8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Engyodontium_album Engyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ID8 OCA].
==Reference==
==Reference==
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What can be done with a good crystal and an accurate beamline?, Wang J, Dauter M, Dauter Z, Acta Crystallogr D Biol Crystallogr. 2006 Dec;62(Pt 12):1475-83. Epub 2006, Nov 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17139083 17139083]
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What can be done with a good crystal and an accurate beamline?, Wang J, Dauter M, Dauter Z, Acta Crystallogr D Biol Crystallogr. 2006 Dec;62(Pt 12):1475-83. Epub 2006, Nov 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17139083 17139083]
[[Category: Engyodontium album]]
[[Category: Engyodontium album]]
[[Category: Peptidase K]]
[[Category: Peptidase K]]
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[[Category: proteinase]]
[[Category: proteinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:51:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:28:06 2008''

Revision as of 15:28, 20 March 2008

Image:2id8.gif


PDB ID 2id8

Drag the structure with the mouse to rotate
, resolution 1.27Å
Ligands: , , and
Activity: Peptidase K, with EC number 3.4.21.64
Coordinates: save as pdb, mmCIF, xml



Crystal structure of Proteinase K


Overview

X-ray single-wavelength anomalous diffraction (SAD) data from a crystal of proteinase K were collected using synchrotron radiation of 0.98 A wavelength at SER-CAT 22-ID beamline, Advanced Photon Source, Argonne National Laboratory. At this wavelength, the expected Bijvoet ratio resulting from the presence of one calcium, one chloride and ten S atoms in the 279-residue protein is extremely small at approximately 0.46%. The direct-methods program SHELXD located 11 anomalous sites using data truncated to 2 A resolution. SHELXE was used to produce an easily interpretable electron-density map. This study shows that an accurate beamline and a good-quality crystal provide the possibility of successfully using a very weak anomalous signal of sulfur measured at a short wavelength for phasing a protein structure, even if a small degree of radiation damage is present.

About this Structure

2ID8 is a Single protein structure of sequence from Engyodontium album. Full crystallographic information is available from OCA.

Reference

What can be done with a good crystal and an accurate beamline?, Wang J, Dauter M, Dauter Z, Acta Crystallogr D Biol Crystallogr. 2006 Dec;62(Pt 12):1475-83. Epub 2006, Nov 23. PMID:17139083

Page seeded by OCA on Thu Mar 20 17:28:06 2008

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