2iyf

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[[Image:2iyf.gif|left|200px]]<br /><applet load="2iyf" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2iyf.gif|left|200px]]
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caption="2iyf, resolution 1.70&Aring;" />
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'''THE CRYSTAL STRUCTURE OF MACROLIDE GLYCOSYLTRANSFERASES: A BLUEPRINT FOR ANTIBIOTIC ENGINEERING'''<br />
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{{Structure
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|PDB= 2iyf |SIZE=350|CAPTION= <scene name='initialview01'>2iyf</scene>, resolution 1.70&Aring;
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|SITE= <scene name='pdbsite=AC1:Ery+Binding+Site+For+Residue+A+1400'>AC1</scene>, <scene name='pdbsite=AC2:Udp+Binding+Site+For+Residue+A+1401'>AC2</scene>, <scene name='pdbsite=AC3:Ery+Binding+Site+For+Residue+B+1399'>AC3</scene>, <scene name='pdbsite=AC4:Udp+Binding+Site+For+Residue+B+1400'>AC4</scene> and <scene name='pdbsite=AC5:Mg+Binding+Site+For+Residue+B+1401'>AC5</scene>
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|LIGAND= <scene name='pdbligand=ERY:ERYTHROMYCIN+A'>ERY</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=UDP:URIDINE-5'-DIPHOSPHATE'>UDP</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''THE CRYSTAL STRUCTURE OF MACROLIDE GLYCOSYLTRANSFERASES: A BLUEPRINT FOR ANTIBIOTIC ENGINEERING'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2IYF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_antibioticus Streptomyces antibioticus] with <scene name='pdbligand=ERY:'>ERY</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=UDP:'>UDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Ery+Binding+Site+For+Residue+A+1400'>AC1</scene>, <scene name='pdbsite=AC2:Udp+Binding+Site+For+Residue+A+1401'>AC2</scene>, <scene name='pdbsite=AC3:Ery+Binding+Site+For+Residue+B+1399'>AC3</scene>, <scene name='pdbsite=AC4:Udp+Binding+Site+For+Residue+B+1400'>AC4</scene> and <scene name='pdbsite=AC5:Mg+Binding+Site+For+Residue+B+1401'>AC5</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IYF OCA].
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2IYF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_antibioticus Streptomyces antibioticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IYF OCA].
==Reference==
==Reference==
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The crystal structure of two macrolide glycosyltransferases provides a blueprint for host cell antibiotic immunity., Bolam DN, Roberts S, Proctor MR, Turkenburg JP, Dodson EJ, Martinez-Fleites C, Yang M, Davis BG, Davies GJ, Gilbert HJ, Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5336-41. Epub 2007 Mar 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17376874 17376874]
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The crystal structure of two macrolide glycosyltransferases provides a blueprint for host cell antibiotic immunity., Bolam DN, Roberts S, Proctor MR, Turkenburg JP, Dodson EJ, Martinez-Fleites C, Yang M, Davis BG, Davies GJ, Gilbert HJ, Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5336-41. Epub 2007 Mar 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17376874 17376874]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Streptomyces antibioticus]]
[[Category: Streptomyces antibioticus]]
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[[Category: transferase]]
[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:57:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:35:02 2008''

Revision as of 15:35, 20 March 2008

Image:2iyf.gif


PDB ID 2iyf

Drag the structure with the mouse to rotate
, resolution 1.70Å
Sites: , , , and
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



THE CRYSTAL STRUCTURE OF MACROLIDE GLYCOSYLTRANSFERASES: A BLUEPRINT FOR ANTIBIOTIC ENGINEERING


Overview

Glycosylation of macrolide antibiotics confers host cell immunity from endogenous and exogenous agents. The Streptomyces antibioticus glycosyltransferases, OleI and OleD, glycosylate and inactivate oleandomycin and diverse macrolides including erythromycin, respectively. The structure of these enzyme-ligand complexes, in tandem with kinetic analysis of site-directed variants, provide insight into the interaction of macrolides with their synthetic apparatus. Erythromycin binds to OleD and the 23S RNA of its target ribosome in the same conformation and, although the antibiotic contains a large number of polar groups, its interaction with these macromolecules is primarily through hydrophobic contacts. Erythromycin and oleandomycin, when bound to OleD and OleI, respectively, adopt different conformations, reflecting a subtle effect on sugar positioning by virtue of a single change in the macrolide backbone. The data reported here provide structural insight into the mechanism of resistance to both endogenous and exogenous antibiotics, and will provide a platform for the future redesign of these catalysts for antibiotic remodelling.

About this Structure

2IYF is a Single protein structure of sequence from Streptomyces antibioticus. Full crystallographic information is available from OCA.

Reference

The crystal structure of two macrolide glycosyltransferases provides a blueprint for host cell antibiotic immunity., Bolam DN, Roberts S, Proctor MR, Turkenburg JP, Dodson EJ, Martinez-Fleites C, Yang M, Davis BG, Davies GJ, Gilbert HJ, Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5336-41. Epub 2007 Mar 21. PMID:17376874

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