2jxm
From Proteopedia
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| - | [[Image:2jxm.jpg|left|200px]]< | + | [[Image:2jxm.jpg|left|200px]] |
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| - | '''Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex''' | + | {{Structure |
| + | |PDB= 2jxm |SIZE=350|CAPTION= <scene name='initialview01'>2jxm</scene> | ||
| + | |SITE= <scene name='pdbsite=AC1:Cu+Binding+Site+For+Residue+A+98'>AC1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene> and <scene name='pdbligand=HEC:HEME C'>HEC</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= petE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1223 Prochlorothrix hollandica]), petA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1223 Prochlorothrix hollandica]) | ||
| + | }} | ||
| + | |||
| + | '''Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2JXM is a [ | + | 2JXM is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Prochlorothrix_hollandica Prochlorothrix hollandica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JXM OCA]. |
==Reference== | ==Reference== | ||
| - | Dynamics in the transient complex of plastocyanin-cytochrome f from Prochlorothrix hollandica., Hulsker R, Baranova MV, Bullerjahn GS, Ubbink M, J Am Chem Soc. 2008 Feb 13;130(6):1985-91. Epub 2008 Jan 18. PMID:[http:// | + | Dynamics in the transient complex of plastocyanin-cytochrome f from Prochlorothrix hollandica., Hulsker R, Baranova MV, Bullerjahn GS, Ubbink M, J Am Chem Soc. 2008 Feb 13;130(6):1985-91. Epub 2008 Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18201089 18201089] |
[[Category: Prochlorothrix hollandica]] | [[Category: Prochlorothrix hollandica]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: transport]] | [[Category: transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:45:48 2008'' |
Revision as of 15:45, 20 March 2008
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| Sites: | |||||||
| Ligands: | and | ||||||
| Gene: | petE (Prochlorothrix hollandica), petA (Prochlorothrix hollandica) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex
Overview
The nature of transient protein complexes can range from a highly dynamic ensemble of orientations to a single well-defined state. This represents variation in the equilibrium between the encounter and final, functional state. The transient complex between plastocyanin (Pc) and cytochrome f (cytf) of the cyanobacterium Prochlorothrix hollandica was characterized by NMR spectroscopy. Intermolecular pseudocontact shifts and chemical shift perturbations were used as restraints in docking calculations to determine the structure of the wild-type Pc-cytf complex. The orientation of Pc is similar to orientations found in Pc-cytf complexes from other sources. Electrostatics seems to play a modest role in complex formation. A large variability in the ensemble of lowest energy structures indicates a dynamic nature of the complex. Two unusual hydrophobic patch residues in Pc have been mutated to the residues found in other plastocyanins (Y12G/P14L). The binding constants are similar for the complexes of cytf with wild-type Pc and mutant Pc, but the chemical shift perturbations are smaller for the complex with mutant Pc. Docking calculations for the Y12G/P14L Pc-cytf complex did not produce a converged ensemble of structures. Simulations of the dynamics were performed using the observed averaged NMR parameters as input. The results indicate a surprisingly large amplitude of mobility of Y12G/P14L Pc within the complex. It is concluded that the double mutation shifts the complex further from the well-defined toward the encounter state.
About this Structure
2JXM is a Protein complex structure of sequences from Prochlorothrix hollandica. Full crystallographic information is available from OCA.
Reference
Dynamics in the transient complex of plastocyanin-cytochrome f from Prochlorothrix hollandica., Hulsker R, Baranova MV, Bullerjahn GS, Ubbink M, J Am Chem Soc. 2008 Feb 13;130(6):1985-91. Epub 2008 Jan 18. PMID:18201089
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