2nov
From Proteopedia
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| - | [[Image:2nov.gif|left|200px]] | + | [[Image:2nov.gif|left|200px]] |
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| - | '''Breakage-reunion domain of S.pneumoniae topo IV: crystal structure of a gram-positive quinolone target''' | + | {{Structure |
| + | |PDB= 2nov |SIZE=350|CAPTION= <scene name='initialview01'>2nov</scene>, resolution 2.67Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= parC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 Streptococcus pneumoniae]) | ||
| + | }} | ||
| + | |||
| + | '''Breakage-reunion domain of S.pneumoniae topo IV: crystal structure of a gram-positive quinolone target''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2NOV is a [ | + | 2NOV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NOV OCA]. |
==Reference== | ==Reference== | ||
| - | Breakage-reunion domain of Streptococcus pneumoniae topoisomerase IV: crystal structure of a gram-positive quinolone target., Laponogov I, Veselkov DA, Sohi MK, Pan XS, Achari A, Yang C, Ferrara JD, Fisher LM, Sanderson MR, PLoS ONE. 2007 Mar 21;2(3):e301. PMID:[http:// | + | Breakage-reunion domain of Streptococcus pneumoniae topoisomerase IV: crystal structure of a gram-positive quinolone target., Laponogov I, Veselkov DA, Sohi MK, Pan XS, Achari A, Yang C, Ferrara JD, Fisher LM, Sanderson MR, PLoS ONE. 2007 Mar 21;2(3):e301. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17375187 17375187] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptococcus pneumoniae]] | [[Category: Streptococcus pneumoniae]] | ||
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[[Category: topo iv]] | [[Category: topo iv]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:49:07 2008'' |
Revision as of 15:49, 20 March 2008
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| , resolution 2.67Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | parC (Streptococcus pneumoniae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Breakage-reunion domain of S.pneumoniae topo IV: crystal structure of a gram-positive quinolone target
Overview
The 2.7 A crystal structure of the 55-kDa N-terminal breakage-reunion domain of topoisomerase (topo) IV subunit A (ParC) from Streptococcus pneumoniae, the first for the quinolone targets from a gram-positive bacterium, has been solved and reveals a 'closed' dimer similar in fold to Escherichia coli DNA gyrase subunit A (GyrA), but distinct from the 'open' gate structure of Escherichia coli ParC. Unlike GyrA whose DNA binding groove is largely positively charged, the DNA binding site of ParC exhibits a distinct pattern of alternating positively and negatively charged regions coincident with the predicted positions of the grooves and phosphate backbone of DNA. Based on the ParC structure, a new induced-fit model for sequence-specific recognition of the gate (G) segment by ParC has been proposed. These features may account for the unique DNA recognition and quinolone targeting properties of pneumococcal type II topoisomerases compared to their gram-negative counterparts.
About this Structure
2NOV is a Single protein structure of sequence from Streptococcus pneumoniae. Full crystallographic information is available from OCA.
Reference
Breakage-reunion domain of Streptococcus pneumoniae topoisomerase IV: crystal structure of a gram-positive quinolone target., Laponogov I, Veselkov DA, Sohi MK, Pan XS, Achari A, Yang C, Ferrara JD, Fisher LM, Sanderson MR, PLoS ONE. 2007 Mar 21;2(3):e301. PMID:17375187
Page seeded by OCA on Thu Mar 20 17:49:07 2008
