2o7c

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[[Image:2o7c.jpg|left|200px]]<br /><applet load="2o7c" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2o7c.jpg|left|200px]]
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caption="2o7c, resolution 1.7&Aring;" />
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'''Crystal structure of L-methionine-lyase from Pseudomonas'''<br />
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{{Structure
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|PDB= 2o7c |SIZE=350|CAPTION= <scene name='initialview01'>2o7c</scene>, resolution 1.7&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Methionine_gamma-lyase Methionine gamma-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.11 4.4.1.11]
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|GENE=
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}}
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'''Crystal structure of L-methionine-lyase from Pseudomonas'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2O7C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Methionine_gamma-lyase Methionine gamma-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.11 4.4.1.11] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7C OCA].
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2O7C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O7C OCA].
==Reference==
==Reference==
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Structure of the antitumour enzyme L-methionine gamma-lyase from Pseudomonas putida at 1.8 A resolution., Kudou D, Misaki S, Yamashita M, Tamura T, Takakura T, Yoshioka T, Yagi S, Hoffman RM, Takimoto A, Esaki N, Inagaki K, J Biochem. 2007 Apr;141(4):535-44. Epub 2007 Feb 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17289792 17289792]
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Structure of the antitumour enzyme L-methionine gamma-lyase from Pseudomonas putida at 1.8 A resolution., Kudou D, Misaki S, Yamashita M, Tamura T, Takakura T, Yoshioka T, Yagi S, Hoffman RM, Takimoto A, Esaki N, Inagaki K, J Biochem. 2007 Apr;141(4):535-44. Epub 2007 Feb 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17289792 17289792]
[[Category: Methionine gamma-lyase]]
[[Category: Methionine gamma-lyase]]
[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
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[[Category: plp]]
[[Category: plp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:15:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:56:02 2008''

Revision as of 15:56, 20 March 2008

Image:2o7c.jpg


PDB ID 2o7c

Drag the structure with the mouse to rotate
, resolution 1.7Å
Ligands:
Activity: Methionine gamma-lyase, with EC number 4.4.1.11
Coordinates: save as pdb, mmCIF, xml



Crystal structure of L-methionine-lyase from Pseudomonas


Overview

l-Methionine gamma-lyase (EC 4.4.1.11, MGL_Pp) from Pseudomonas putida is a multifunctional enzyme, which belongs to the gamma-family of pyridoxal-5'-phosphate (PLP) dependent enzymes. In this report, we demonstrate that the three-dimensional structure of MGL_Pp has been completely solved by the molecular replacement method to an R-factor of 20.4% at 1.8 A resolution. Detailed information of the overall structure of MGL_Pp supplies a clear picture of the substrate- and PLP-binding pockets. Tyr59 and Arg61 of neighbouring subunits, which are strongly conserved in other gamma-family enzymes, contact the phosphate group of PLP. These residues are important as the main anchor within the active site. Lys240, Asp241 and Arg61 of one partner monomer and Tyr114 and Cys116 of the other partner monomer form a hydrogen-bond network in the MGL active site which is specific for MGLs. It is also suggested that electrostatic interactions at the subunit interface are involved in the stabilization of the structural conformation. The detailed structure will facilitate the development of MGL_Pp as an anticancer drug.

About this Structure

2O7C is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

Structure of the antitumour enzyme L-methionine gamma-lyase from Pseudomonas putida at 1.8 A resolution., Kudou D, Misaki S, Yamashita M, Tamura T, Takakura T, Yoshioka T, Yagi S, Hoffman RM, Takimoto A, Esaki N, Inagaki K, J Biochem. 2007 Apr;141(4):535-44. Epub 2007 Feb 8. PMID:17289792

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