Galactosidase
From Proteopedia
(Difference between revisions)
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**[[1uas]] – Agal – rice<br /> | **[[1uas]] – Agal – rice<br /> | ||
**[[2xn0]], [[2xn1]] – LaAgal – ''Lactobacillus acidophilus''<br /> | **[[2xn0]], [[2xn1]] – LaAgal – ''Lactobacillus acidophilus''<br /> | ||
| - | **[[4fnp]] – GsAgal (mutant) – ''Geobacillus stearothermophilus''<br /> | + | **[[4fnp]], [[4ojy]] – GsAgal (mutant) – ''Geobacillus stearothermophilus''<br /> |
| - | **[[4fnq]], [[4fnr]] – GsAgal | + | **[[4fnq]], [[4fnr]], [[4oif]] – GsAgal |
*''α-galactosidase binary complex'' | *''α-galactosidase binary complex'' | ||
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**[[3hg4]] – hAgal A + intermediate<br /> | **[[3hg4]] – hAgal A + intermediate<br /> | ||
**[[3gxt]] – hAgal A + imino sugar<br /> | **[[3gxt]] – hAgal A + imino sugar<br /> | ||
| + | **[[4nxs]] - hAgal + jirimycin derivative<br /> | ||
**[[1t0o]] – HjAgal + galactose<br /> | **[[1t0o]] – HjAgal + galactose<br /> | ||
**[[2xn2]] - LaAgal + galactose<br /> | **[[2xn2]] - LaAgal + galactose<br /> | ||
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**[[3og2]] – TrBgal – ''Trichoderma reesei''<br /> | **[[3og2]] – TrBgal – ''Trichoderma reesei''<br /> | ||
**[[3muy]], [[3i3e]], [[3iap]], [[3iaq]], [[3dyp]], [[1px3]], [[3sep]], [[3t0a]], [[3t2o]], [[3vd3]], [[3vd5]], [[3vda]], [[4duv]] – EcBgal (mutant) – ''Escherichia coli''<br /> | **[[3muy]], [[3i3e]], [[3iap]], [[3iaq]], [[3dyp]], [[1px3]], [[3sep]], [[3t0a]], [[3t2o]], [[3vd3]], [[3vd5]], [[3vda]], [[4duv]] – EcBgal (mutant) – ''Escherichia coli''<br /> | ||
| - | **[[1hn1]], [[1dp0]], [[1f49]], [[1f4a]], [[1f4h]], [[1gho]], [[1bgl]], [[1bgm]] - EcBgal<br /> | + | **[[1hn1]], [[1dp0]], [[1f49]], [[1f4a]], [[1f4h]], [[1gho]], [[1bgl]], [[1bgm]], [[4ttg]] - EcBgal<br /> |
| + | **[[3j7h]] – EcBgal – cryo EM<br /> | ||
**[[3dec]], [[3d3a]], [[3bga]] – BtBgal<br /> | **[[3dec]], [[3d3a]], [[3bga]] – BtBgal<br /> | ||
**[[1yq2]] - Bgal (mutant) – Arthrobacter<br /> | **[[1yq2]] - Bgal (mutant) – Arthrobacter<br /> | ||
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**[[4ha3]], [[4ha4]] – Bgal – ''Acidilobus saccharovorans''<br /> | **[[4ha3]], [[4ha4]] – Bgal – ''Acidilobus saccharovorans''<br /> | ||
**[[3w5f]] – toBgal – tomato<br /> | **[[3w5f]] – toBgal – tomato<br /> | ||
| + | **[[3wq8]] – Bgal – ''Pyrococcus furiosus''<br /> | ||
| + | **[[4cu6]] – SpBgal catalytic module residues 137-985– ''Streptococcus pneumoniae''<br /> | ||
| + | **[[4cu9]] – SpBgal carbohydrate-binding module II residues 1822-1998 <br /> | ||
| + | **[[4cua]] – SpBgal carbohydrate-binding module I residues 1463-1645 <br /> | ||
| + | **[[4uoq]] - BaBgal (mutant) – ''Bifidobacterium animalis''<br /> | ||
| + | **[[3ob8]], [[3oba]] – Bgal – ''Kluyveromyces lactis''<br /> | ||
| - | *''β-Galactosidase binary complex'' | + | *''β-Galactosidase binary complex with galactose'' |
**[[1xc6]] - PeBgal + galactose<br /> | **[[1xc6]] - PeBgal + galactose<br /> | ||
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**[[1kwk]] – TtBgal + galactose<br /> | **[[1kwk]] – TtBgal + galactose<br /> | ||
**[[3ogr]] – TrBgal + galactose<br /> | **[[3ogr]] – TrBgal + galactose<br /> | ||
| + | **[[3e1f]], [[1jz7]] - EcBgal (mutant) + galactose<br /> | ||
| + | **[[3t09]], [[3t0d]], [[3t2q]], [[3vd7]], [[3vdb]] - EcBgal (mutant) + galactose derivative<br /> | ||
| + | **[[4iug]] - Bgal + galactose – ''Aspergillus oryzae''<br /> | ||
| + | **[[3tty]] - BcBgal + galactose<br /> | ||
| + | **[[3thc]] - hBgal + galactose<br /> | ||
| + | **[[3wf3]] - hBgal + galactose + NAG<br /> | ||
| + | **[[4cub]] – SpBgal carbohydrate-binding module I + galactose <br /> | ||
| + | **[[4cuc]] – SpBgal catalytic module (mutant) + galactose <br /> | ||
| + | **[[4uni]] - BaBgal + galactose <br /> | ||
| + | **[[4uoz]] - BaBgal (mutant) + galactose <br /> | ||
| + | |||
| + | *''β-Galactosidase other complexes'' | ||
| + | |||
**[[3ogs]] - TrBgal + IPTG<br /> | **[[3ogs]] - TrBgal + IPTG<br /> | ||
**[[3ogv]] – TrBgal + inhibitor<br /> | **[[3ogv]] – TrBgal + inhibitor<br /> | ||
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**[[3mv0]], [[3i3b]], [[3czj]], [[1jz0]], [[1jz1]], [[1jz2]], [[1jz3]], [[1jz4]], [[1jz5]], [[1jz6]] - EcBgal (mutant) + intermediate<br /> | **[[3mv0]], [[3i3b]], [[3czj]], [[1jz0]], [[1jz1]], [[1jz2]], [[1jz3]], [[1jz4]], [[1jz5]], [[1jz6]] - EcBgal (mutant) + intermediate<br /> | ||
**[[3mv1]] - EcBgal (mutant) + guanidinium<br /> | **[[3mv1]] - EcBgal (mutant) + guanidinium<br /> | ||
| - | **[[3e1f]], [[1jz7]] - EcBgal (mutant) + galactose<br /> | ||
| - | **[[3t09]], [[3t0d]], [[3t2q]], [[3vd7]], [[3vdb]] - EcBgal (mutant) + galactose derivative<br /> | ||
**[[1jyn]], [[1jyy]], [[1jyz]], [[1jz8]] - EcBgal (mutant) + lactose derivative<br /> | **[[1jyn]], [[1jyy]], [[1jyz]], [[1jz8]] - EcBgal (mutant) + lactose derivative<br /> | ||
**[[1jyn]], [[1jyw]], [[1jyv]] - EcBgal (mutant) + substrate<br /> | **[[1jyn]], [[1jyw]], [[1jyv]] - EcBgal (mutant) + substrate<br /> | ||
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**[[4dux]] - EcBgal (mutant) + ribose<br /> | **[[4dux]] - EcBgal (mutant) + ribose<br /> | ||
**[[4ckd]] – EcBgal + antibody<br /> | **[[4ckd]] – EcBgal + antibody<br /> | ||
| - | **[[4iug]] - Bgal + galactose – ''Aspergillus oryzae''<br /> | ||
| - | **[[3ob8]], [[3oba]] – Bgal – ''Kluyveromyces lactis''<br /> | ||
| - | **[[3tty]] - BcBgal + galactose<br /> | ||
**[[3thd]] – hBgal + jirimycin derivative<br /> | **[[3thd]] – hBgal + jirimycin derivative<br /> | ||
| - | **[[ | + | **[[3wez]] - hBgal + NOEV<br /> |
| + | **[[3wf0]], [[3wf1]] - hBgal + pyrimidine derivative<br /> | ||
| + | **[[3wf2]] - hBgal + piperidine derivative<br /> | ||
| + | **[[4cu7]] - SpBgal catalytic module + galacto-isofagomine<br /> | ||
*Endo-1,4-β-galactosidase | *Endo-1,4-β-galactosidase | ||
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**[[2xom]], [[2xon]] – TmEbgal carbohydrate-binding domain + β-galactotriose<br /> | **[[2xom]], [[2xon]] – TmEbgal carbohydrate-binding domain + β-galactotriose<br /> | ||
**[[1ups]] – Ebgal - ''Clostridium perfringens''<br /> | **[[1ups]] – Ebgal - ''Clostridium perfringens''<br /> | ||
| - | **[[2ygl]], [[2ygm]] – | + | **[[2ygl]], [[2ygm]] – SpEbgal blood group A & B cleaving <br /> |
| - | **[[ | + | **[[4bf7]] – Ebgal – ''Emericella nidulans''<br /> |
*3,6-anhydro-α-L-galactosidase | *3,6-anhydro-α-L-galactosidase | ||
Revision as of 06:54, 6 April 2015
α-Galactosidase (Agal) hydrolyzes the terminal α-galactosyl moiety from glycoproteins and glycolipids. β-Galactosidase (Bgal) hydrolyzes β-galactosides into monosaccharides. Isopropyl-β-d-thiogalactopyranoside (IPTG) induces Bgal activity. Phenylethyl-β-d-thiogalactopyranoside (PETG) is an inhibitor. Galactose, lactose, o-nitrophenyl-β-d-galactoside (ONPG) are substrates. Galactopyranosyl is reaction intermediate. The image at the right correspond to one representative galactosidase, i.e. the crystal structure of galactosidase from Escherichia coli (3e1f). For details on β-galactosidase see Molecular Playground/Beta-galactosidase.
3D Structures of Galactosidase
Updated on 06-April-2015
For 3hg2 .
- Created with the participation of Wayne Decatur.
