2tnf

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[[Image:2tnf.gif|left|200px]]<br /><applet load="2tnf" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2tnf.gif|left|200px]]
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caption="2tnf, resolution 1.40&Aring;" />
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'''1.4 A RESOLUTION STRUCTURE OF MOUSE TUMOR NECROSIS FACTOR, TOWARDS MODULATION OF ITS SELCTIVITY AND TRIMERISATION'''<br />
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{{Structure
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|PDB= 2tnf |SIZE=350|CAPTION= <scene name='initialview01'>2tnf</scene>, resolution 1.40&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene> and <scene name='pdbligand=IPA:ISOPROPYL ALCOHOL'>IPA</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''1.4 A RESOLUTION STRUCTURE OF MOUSE TUMOR NECROSIS FACTOR, TOWARDS MODULATION OF ITS SELCTIVITY AND TRIMERISATION'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2TNF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=TRS:'>TRS</scene> and <scene name='pdbligand=IPA:'>IPA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TNF OCA].
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2TNF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TNF OCA].
==Reference==
==Reference==
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The structure of mouse tumour-necrosis factor at 1.4 A resolution: towards modulation of its selectivity and trimerization., Baeyens KJ, De Bondt HL, Raeymaekers A, Fiers W, De Ranter CJ, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):772-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10089307 10089307]
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The structure of mouse tumour-necrosis factor at 1.4 A resolution: towards modulation of its selectivity and trimerization., Baeyens KJ, De Bondt HL, Raeymaekers A, Fiers W, De Ranter CJ, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):772-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10089307 10089307]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: transmembrane]]
[[Category: transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:49:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:39:13 2008''

Revision as of 16:39, 20 March 2008

Image:2tnf.gif


PDB ID 2tnf

Drag the structure with the mouse to rotate
, resolution 1.40Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



1.4 A RESOLUTION STRUCTURE OF MOUSE TUMOR NECROSIS FACTOR, TOWARDS MODULATION OF ITS SELCTIVITY AND TRIMERISATION


Overview

The 1.4 A resolution structure of recombinant mouse tumour-necrosis factor alpha (mTNF) at 100 K has been determined. The crystals are triclinic, space group P1, with unit-cell parameters a = 48.06, b = 48.18, c = 51.01 A, alpha = 114.8, beta = 103.6, gamma = 91.1 degrees. The structure was refined to a final crystallographic R value of 19.7% (Rfree = 23.3%), including 3477 protein atoms, one 2-propanol molecule, one Tris molecule and 240 water molecules. Throughout the crystal lattice, the trimers are differently packed compared with human TNF, which was crystallized in the tetragonal space group P41212 and refined to 2.6 A resolution. The structures of mTNF and human TNF are very similar, diverging mainly in regions that are either flexible and/or involved in crystal packing. Some loops in mTNF which contain residues important for receptor binding are better resolved than in human TNF, such as the surface-exposed loops 30-34 and 144-147, which are also important for receptor specificity. Compared with human TNFs, the channel formed by the three monomers in mTNF is narrower. One 2-propanol molecule trapped in the trimeric channel could be a lead compound for the design of TNF inhibitors.

About this Structure

2TNF is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

The structure of mouse tumour-necrosis factor at 1.4 A resolution: towards modulation of its selectivity and trimerization., Baeyens KJ, De Bondt HL, Raeymaekers A, Fiers W, De Ranter CJ, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):772-8. PMID:10089307

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