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Structural Highlights

Shiga toxin (stx) has multiple classifications. The shiga toxin produced by Shigella dysenteriae is classified as stx, while shiga toxin produced by Escherichia coli can be classified as either stx1 or stx2. All three classifications very slightly in structure but have identical functions. The A sub-unit of stx1 differs from stx by only one residue: a serine in position 45 as opposed to threonine^[1]. Stx is a 70 kDa AB5 protein, meaning that it is composed of an A sub-unit bound to a B pentamer. A noncovalent interaction causes the association between the A and B sub-units, as the carboxy terminal tail of the A sub-unit is surrounded by the B pentamer. is 293 amino acids long with its active site being . Though the active site is a single residue, studies have shown that the first 239 residues are essential for the enzymatic activity of the A sub-unit. Residues 240-251 are essential for the A sub-unit’s translocation from the endoplasmic reticulum of an infected cell to the cytosol^[2]. The A sub-unit can be broken down into A1 and A2 units. This break down occurs when a trypsin sensitive region, residues 248-251, is cleaved. This cleavage results in the only remaining link being a between cysteine 242 and cysteine 261. If the disulfide bridge is reduced, the A1 and A2 fragments can completely separate. Interestingly, the disulfide bridge blocks the active site of the A sub-unit, so the A sub-unit is not enzymatically active unless it has been cleaved^[1]. is a symmetrical pentamer that resembles a star and can be divided into five identical monomers. Each of the monomers is 69 amino acids in length. The purpose of the B sub-unit is to bind to globotriaosylceramide (GB3) which is a glycosphingolipid that is found on the lipid rafts of endothelial cells. Each monomer of the B sub-unit has three binding sites for GB3, binding to the . The affinity between the B sub-unit and GB3 is incredibly low, and is actually one of the lowest recorded affinities for carbohydrate-protein interaction^[3]. Some studies have shown that the level of saturation and length of the fatty acid on GB3 affects the strength of its interaction with the B sub-unit. Since the B sub-unit of stx has fifteen binding sites though, it has an incredibly high avidity^[4].

Function

The pathway of stx entering a cell begins with the B sub-unit’s binding to GB3. Once this occurs, the A sub-unit disconnects from the B sub-unit and enters the cell through endocytosis. Using retrograde transport the A sub-unit passes through the Golgi apparatus and the rough endoplasmic reticulum^[4] In the rough endoplasmic reticulum, the A sub-unit is split into two parts called A1 and A2 through the cleavage of trypsin sensitive residues and the reduction of a disulfide bridge. A2 is degraded, but A1 freely enters the cytosol^[2]. Once in the cytosol, A1 acts as an N-glycosidase, which is an enzyme that hydrolyzes bonds that link sugars. With this enzymatic activity, A1 removes an adenine from the alpha-sarcin loop in the 28S RNA of the 60S ribosomal sub-unit^[4]. The removal of the adenine prevents elongation factors from associating with the ribosomal sub-unit. Without elongation factors, the ribosome can no longer synthesize proteins, leading to cell death^[4].

Disease and Pathogenesis

The shiga toxin is produced by Shigella dysenteriae and certain strains of Escherichia coli. S. dysenteriae is a gram negative bacterium that causes disease in its host by entering into intestinal epithelial cells. It has a very low infectious dose of only ten to one hundred cells, and is contracted through the consumption of contaminated water or food. One it begins replicating in the intestines, its proliferation and secretion of stx erodes the intestinal lining and leads to dysentery^[5]. Those with dysentery can simply have diarrhea as a symptom, but if the infection progresses, it can lead to hemorrhagic colitis (bloody stool) or hemolytic uremic syndrome (HUS). HUS is characterized as acute renal failure, thrombocytopenia, and microangiopathic anemia^[6]. As stated previously, stx enters cells through its binding to GB3, but curiously many intestinal cells do not express GB3. Some researchers hypothesize it enters the cells through macropinocyosis, but when macropinocytotic function is removed from cells, stx still manages to enter through an undefined mechanism. The genes necessary to produce the toxin are interestingly not native to S. dysenteriae. The genes for stx are carried by prophages: bacteria that have had viral DNA inserted into their genome. During certain conditions, the phage genes are expressed leading to the production of more bacteriophages as well as the shiga toxin. When the bacteria undergo lysis, the bacteriophages and stx are released. It may seem as if there is no benefit for S. dysenteriae to carry the viral DNA, but studies have shown that while it is in the lysogenic cycle S. dysenteriae has increased acid tolerance and motility^[7].