This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2zb9
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2zb9.jpg|left|200px]] | + | [[Image:2zb9.jpg|left|200px]] |
| - | + | ||
| - | '''Crystal structure of TetR family transcription regulator SCO0332''' | + | {{Structure |
| + | |PDB= 2zb9 |SIZE=350|CAPTION= <scene name='initialview01'>2zb9</scene>, resolution 2.25Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= sco0332 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of TetR family transcription regulator SCO0332''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2ZB9 is a [ | + | 2ZB9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZB9 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural and functional analysis of the TetR-family transcriptional regulator SCO0332 from Streptomyces coelicolor., Okada U, Kondo K, Hayashi T, Watanabe N, Yao M, Tamura T, Tanaka I, Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):198-205. Epub 2008, Jan 16. PMID:[http:// | + | Structural and functional analysis of the TetR-family transcriptional regulator SCO0332 from Streptomyces coelicolor., Okada U, Kondo K, Hayashi T, Watanabe N, Yao M, Tamura T, Tanaka I, Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):198-205. Epub 2008, Jan 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18219120 18219120] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces coelicolor]] | [[Category: Streptomyces coelicolor]] | ||
| Line 24: | Line 33: | ||
[[Category: transcription regulator]] | [[Category: transcription regulator]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:53:32 2008'' |
Revision as of 16:53, 20 March 2008
| |||||||
| , resolution 2.25Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | sco0332 (Streptomyces coelicolor) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of TetR family transcription regulator SCO0332
Overview
SCO0332 protein is a putative TetR-family transcriptional regulator from Streptomyces coelicolor A3(2). The crystal structure of SCO0332 was determined at 2.25 A resolution by single-wavelength anomalous diffraction (SAD) phasing using the S atoms of the native protein. SCO0332 contains a helix-turn-helix (HTH) DNA-binding motif in its N-terminal region and forms a homodimer. The overall structure of SCO0332 shows significant similarity to other TetR-family regulators. A systematic evolution of ligands by exponential enrichment (SELEX) analysis indicated that SCO0332 has sequence-specific DNA-binding ability and determined the position of the operator element of SCO0332 on the chromosomal DNA of S. coelicolor. An electrophoretic mobility-shift assay (EMSA) showed that SCO0332 binds to the operator sequence upstream of the sco0330 gene, which encodes a putative short-chain oxidoreductase. These results suggest that SCO0332 is a transcriptional repressor that regulates sco0330 gene expression.
About this Structure
2ZB9 is a Single protein structure of sequence from Streptomyces coelicolor. Full crystallographic information is available from OCA.
Reference
Structural and functional analysis of the TetR-family transcriptional regulator SCO0332 from Streptomyces coelicolor., Okada U, Kondo K, Hayashi T, Watanabe N, Yao M, Tamura T, Tanaka I, Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):198-205. Epub 2008, Jan 16. PMID:18219120
Page seeded by OCA on Thu Mar 20 18:53:32 2008
