4zk7

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'''Unreleased structure'''
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==Crystal structure of rescued two-component self-assembling tetrahedral cage T33-31==
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<StructureSection load='4zk7' size='340' side='right' caption='[[4zk7]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4zk7]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZK7 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zk7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4zk7 RCSB], [http://www.ebi.ac.uk/pdbsum/4zk7 PDBsum]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/CUTA_THET8 CUTA_THET8]] Involved in resistance toward heavy metals (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We recently reported the development of a computational method for the design of co-assembling, multi-component protein nanomaterials. While four such materials were validated at high-resolution by X-ray crystallography, low yield of soluble protein prevented X-ray structure determination of a fifth designed material, T33-09. Here we report the design and crystal structure of T33-31, a variant of T33-09 with improved soluble yield resulting from redesign efforts focused on mutating solvent-exposed side chains to charged amino acids. The structure is found to match the computational design model with atomic-level accuracy, providing further validation of the design approach and demonstrating a simple and potentially general means of improving the yield of designed protein nanomaterials. This article is protected by copyright. All rights reserved.
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The entry 4zk7 is ON HOLD until Paper Publication
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Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression.,Bale JB, Park RU, Liu Y, Gonen S, Gonen T, Cascio D, King NP, Yeates TO, Baker D Protein Sci. 2015 Jul 15. doi: 10.1002/pro.2748. PMID:26174163<ref>PMID:26174163</ref>
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Authors: Liu, Y., Cascio, D., Sawaya, M.R., Bale, J., Collazo, M.J., Park, R., King, N., Baker, D., Yeates, T.
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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Description: Crystal structure of rescued two-component self-assembling tetrahedral cage T33-31
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== References ==
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[[Category: Unreleased Structures]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Baker, D]]
[[Category: Bale, J]]
[[Category: Bale, J]]
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[[Category: Sawaya, M.R]]
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[[Category: Cascio, D]]
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[[Category: Collazo, M J]]
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[[Category: King, N]]
[[Category: Liu, Y]]
[[Category: Liu, Y]]
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[[Category: Baker, D]]
 
[[Category: Park, R]]
[[Category: Park, R]]
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[[Category: King, N]]
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[[Category: Sawaya, M R]]
[[Category: Yeates, T]]
[[Category: Yeates, T]]
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[[Category: Cascio, D]]
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[[Category: Computational design]]
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[[Category: Collazo, M.J]]
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[[Category: Nanomaterial]]
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[[Category: Protein binding]]
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[[Category: Rosetta]]
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[[Category: Self-assembly]]
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[[Category: Solubility]]
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[[Category: Symmetric]]
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[[Category: Tetrahedral]]

Revision as of 14:38, 29 July 2015

Crystal structure of rescued two-component self-assembling tetrahedral cage T33-31

4zk7, resolution 3.40Å

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