1m32
From Proteopedia
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|PDB= 1m32 |SIZE=350|CAPTION= <scene name='initialview01'>1m32</scene>, resolution 2.2Å | |PDB= 1m32 |SIZE=350|CAPTION= <scene name='initialview01'>1m32</scene>, resolution 2.2Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5 | + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5'-PHOSPHATE'>PLP</scene> and <scene name='pdbligand=POA:PHOSPHONOACETALDEHYDE'>POA</scene> |
|ACTIVITY= [http://en.wikipedia.org/wiki/2-aminoethylphosphonate--pyruvate_transaminase 2-aminoethylphosphonate--pyruvate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.37 2.6.1.37] | |ACTIVITY= [http://en.wikipedia.org/wiki/2-aminoethylphosphonate--pyruvate_transaminase 2-aminoethylphosphonate--pyruvate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.37 2.6.1.37] | ||
|GENE= | |GENE= | ||
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[[Category: plp-dependent aminotransferase fold]] | [[Category: plp-dependent aminotransferase fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 12:42:35 2008'' |
Revision as of 10:42, 23 March 2008
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| , resolution 2.2Å | |||||||
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| Ligands: | , and | ||||||
| Activity: | 2-aminoethylphosphonate--pyruvate transaminase, with EC number 2.6.1.37 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of 2-aminoethylphosphonate Transaminase
Overview
Phosphonates allow certain organisms to thrive in otherwise hostile environments, and 2-aminoethylphosphonate (AEP) is a precursor of many cellular phosphonates. AEP transaminase (AEPT) is an enzyme essential to phosphonate synthesis and degradation pathways. The crystal structure of AEP transaminase was determined by multiwavelength anomalous diffraction of 66 selenium atoms. The refined structure at 2.2 A resolution revealed an overall fold and active site location similar to those of the dimeric, two-domain structure of type I aminotransferases. The active site contains a cofactor, pyridoxal 5'-phosphate (PLP), and the product phosphonoacetaldehyde. Comparison with other type I aminotransferase structures shows that the PLP-protein interactions are conserved. Modeling of bound substrates and products reveals the structural basis for AEP recognition and the stereospecificity of proton elimination at the alpha-carbon and indicates conformational changes along the reaction pathway.
About this Structure
1M32 is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.
Reference
Degradation pathway of the phosphonate ciliatine: crystal structure of 2-aminoethylphosphonate transaminase., Chen CC, Zhang H, Kim AD, Howard A, Sheldrick GM, Mariano-Dunaway D, Herzberg O, Biochemistry. 2002 Nov 5;41(44):13162-9. PMID:12403617
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