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2qe7

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Revision as of 03:38, 10 September 2015

Crystal structure of the f1-atpase from the thermoalkaliphilic bacterium bacillus sp. ta2.a1

Structural highlights

2qe7 is a 8 chain structure with sequence from Bacillus sp. ta2.a1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1jnv, 1sky
Gene:	atpA (Bacillus sp. TA2.A1), atpD (Bacillus sp. TA2.A1), atpG (Bacillus sp. TA2.A1), atpC (Bacillus sp. TA2.A1)
Activity:	H(+)-transporting two-sector ATPase, with EC number 3.6.3.14
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[Q71CG2_9BACI] Produces ATP from ADP in the presence of a proton gradient across the membrane (By similarity).[HAMAP-Rule:MF_00530] [Q71CG5_9BACI] Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit (By similarity).[HAMAP-Rule:MF_01346] [Q71CG4_9BACI] Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex (By similarity).[HAMAP-Rule:MF_00815][RuleBase:RU004001][SAAS:SAAS023632_004_025650] [Q71CG3_9BACI] Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits (By similarity).[HAMAP-Rule:MF_01347]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The ATP synthase of the thermoalkaliphilic Bacillus sp. TA2.A1 operates exclusively in ATP synthesis direction. In the crystal structure of the nucleotide-free alpha(3)beta(3)gamma epsilon subcomplex (TA2F(1)) at 3.1 A resolution, all three beta subunits adopt the open beta(E) conformation. The structure shows salt bridges between the helix-turn-helix motif of the C-terminal domain of the beta(E) subunit (residues Asp372 and Asp375) and the N-terminal helix of the gamma subunit (residues Arg9 and Arg10). These electrostatic forces pull the gamma shaft out of the rotational center and impede rotation through steric interference with the beta(E) subunit. Replacement of Arg9 and Arg10 with glutamines eliminates the salt bridges and results in an activation of ATP hydrolysis activity, suggesting that these salt bridges prevent the native enzyme from rotating in ATP hydrolysis direction. A similar bending of the gamma shaft as in the TA2F(1) structure was observed by single-particle analysis of the TA2F(1)F(o) holoenzyme.

The structural basis for unidirectional rotation of thermoalkaliphilic F1-ATPase.,Stocker A, Keis S, Vonck J, Cook GM, Dimroth P Structure. 2007 Aug;15(8):904-14. PMID:17697996^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Stocker A, Keis S, Vonck J, Cook GM, Dimroth P. The structural basis for unidirectional rotation of thermoalkaliphilic F1-ATPase. Structure. 2007 Aug;15(8):904-14. PMID:17697996 doi:http://dx.doi.org/10.1016/j.str.2007.06.009

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2qe7"

@@ Line 6: / Line 6: @@
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">atpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90973 Bacillus sp. TA2.A1]), atpD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90973 Bacillus sp. TA2.A1]), atpG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90973 Bacillus sp. TA2.A1]), atpC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90973 Bacillus sp. TA2.A1])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qe7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qe7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qe7 RCSB], [http://www.ebi.ac.uk/pdbsum/2qe7 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qe7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qe7 OCA], [http://pdbe.org/2qe7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qe7 RCSB], [http://www.ebi.ac.uk/pdbsum/2qe7 PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 28: / Line 28: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2qe7" style="background-color:#fffaf0;"></div>
 ==See Also==

2qe7

From Proteopedia

Revision as of 03:38, 10 September 2015

Crystal structure of the f1-atpase from the thermoalkaliphilic bacterium bacillus sp. ta2.a1

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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